Localization of human T-cell leukemia virus type 1 tax to subnuclear compartments that overlap with interchromatin speckles
- PMID: 8709263
- PMCID: PMC190661
- DOI: 10.1128/JVI.70.9.6347-6357.1996
Localization of human T-cell leukemia virus type 1 tax to subnuclear compartments that overlap with interchromatin speckles
Abstract
Tax, the virally encoded activator of the human T-cell leukemia virus type 1 long terminal repeats, regulates the expression of many cellular genes. This protein has been implicated in transformation events leading to the development of adult T-cell leukemia. Because subcellular localization contributes importantly to protein function, we determined the compartment(s) within the cell in which Tax is found. Using confocal microscopy, we found that Tax localizes to subnuclear domains which overlap with structures previously identified as interchromatin granules or spliceosomal speckles. These Tax speckled structures are coincident with a subset of nuclear transcriptional hot spots. Disruption of the Tax speckled structures by heat shock revealed the existence of different populations of Tax. One population of Tax is tightly associated with nuclear speckles. A second population exists outside of the speckles and is transcriptionally active for some promoters.
Similar articles
-
Human T-cell leukemia virus type 1 Tax shuttles between functionally discrete subcellular targets.J Virol. 2000 Mar;74(5):2351-64. doi: 10.1128/jvi.74.5.2351-2364.2000. J Virol. 2000. PMID: 10666266 Free PMC article.
-
Suppression of HTLV-1 replication by Tax-mediated rerouting of the p13 viral protein to nuclear speckles.Blood. 2011 Aug 11;118(6):1549-59. doi: 10.1182/blood-2010-06-293340. Epub 2011 Jun 15. Blood. 2011. PMID: 21677314 Free PMC article.
-
Regulation of the human T-cell leukemia virus gene expression depends on the localization of regulatory proteins Tax, Rex and p30II in specific nuclear subdomains.Gene. 2007 Jan 15;386(1-2):191-201. doi: 10.1016/j.gene.2006.09.008. Epub 2006 Sep 30. Gene. 2007. PMID: 17071021
-
[Adult T-cell leukemia induced by HTLV-1: before and after HBZ].Med Sci (Paris). 2010 Apr;26(4):391-6. doi: 10.1051/medsci/2010264391. Med Sci (Paris). 2010. PMID: 20412744 Review. French.
-
Functional activities of the human T-cell leukemia virus type I Tax oncoprotein: cellular signaling through NF-kappa B.Cytokine Growth Factor Rev. 2001 Jun-Sep;12(2-3):207-17. doi: 10.1016/s1359-6101(00)00028-9. Cytokine Growth Factor Rev. 2001. PMID: 11325603 Review.
Cited by
-
Human T-cell leukemia virus type 1 (HTLV-1) tax requires CADM1/TSLC1 for inactivation of the NF-κB inhibitor A20 and constitutive NF-κB signaling.PLoS Pathog. 2015 Mar 16;11(3):e1004721. doi: 10.1371/journal.ppat.1004721. eCollection 2015 Mar. PLoS Pathog. 2015. PMID: 25774694 Free PMC article.
-
HTLV-1 Tax oncoprotein subverts the cellular DNA damage response via binding to DNA-dependent protein kinase.J Biol Chem. 2008 Dec 26;283(52):36311-20. doi: 10.1074/jbc.M804931200. Epub 2008 Oct 27. J Biol Chem. 2008. PMID: 18957425 Free PMC article.
-
Regulation of HTLV-1 tax stability, cellular trafficking and NF-κB activation by the ubiquitin-proteasome pathway.Viruses. 2014 Oct 23;6(10):3925-43. doi: 10.3390/v6103925. Viruses. 2014. PMID: 25341660 Free PMC article. Review.
-
The intricate interplay between RNA viruses and NF-κB.Biochim Biophys Acta. 2014 Nov;1843(11):2754-2764. doi: 10.1016/j.bbamcr.2014.08.004. Epub 2014 Aug 10. Biochim Biophys Acta. 2014. PMID: 25116307 Free PMC article. Review.
-
NF-κB-Induced R-Loops and Genomic Instability in HTLV-1-Infected and Adult T-Cell Leukemia Cells.Viruses. 2022 Apr 23;14(5):877. doi: 10.3390/v14050877. Viruses. 2022. PMID: 35632619 Free PMC article. Review.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
