Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line
- PMID: 8702519
- DOI: 10.1074/jbc.271.31.18657
Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line
Abstract
We have isolated a cDNA from a human placental choriocarcinoma cell cDNA library which, when expressed in HeLa cells, induces a Na+-dependent amino acid transport system with preference for zwitterionic amino acids. Anionic amino acids, cationic amino acids, imino acids, and N-methylated amino acids are excluded by this system. These characteristics are identical to those described for the amino acid transporter Bo. When expressed in Xenopus laevis oocytes that do not have detectable endogenous activity of the amino acid transporter Bo, the cloned transporter increases alanine transport in the oocytes severalfold and induces alanine-evoked inward currents in the presence of Na+. The cDNA codes for a polypeptide containing 541 amino acids with 10 putative transmembrane domains. Amino acid sequence homology predicts this transporter (hATBo) to be a member of a superfamily consisting of the glutamate transporters, the neutral amino acid transport system ASCT, and the insulin-activable neutral/anionic amino acid transporter. Chromosomal assignment studies with somatic cell hybrid analysis and fluorescent in situ hybridization have located the ATBo gene to human chromosome 19q13.3.
Similar articles
-
Molecular and functional characterization of intestinal Na(+)-dependent neutral amino acid transporter B0.Am J Physiol. 1997 Jun;272(6 Pt 1):G1463-72. doi: 10.1152/ajpgi.1997.272.6.G1463. Am J Physiol. 1997. PMID: 9227483
-
Na+- and Cl--coupled active transport of carnitine by the amino acid transporter ATB(0,+) from mouse colon expressed in HRPE cells and Xenopus oocytes.J Physiol. 2001 Apr 15;532(Pt 2):297-304. doi: 10.1111/j.1469-7793.2001.0297f.x. J Physiol. 2001. PMID: 11306651 Free PMC article.
-
Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization.J Biol Chem. 1999 May 21;274(21):14875-83. doi: 10.1074/jbc.274.21.14875. J Biol Chem. 1999. PMID: 10329687
-
Molecular characteristics of mammalian and insect amino acid transporters: implications for amino acid homeostasis.J Exp Biol. 1997 Jan;200(Pt 2):269-86. doi: 10.1242/jeb.200.2.269. J Exp Biol. 1997. PMID: 9050235 Review.
-
Endogenous amino acid transport systems and expression of mammalian amino acid transport proteins in Xenopus oocytes.Biochim Biophys Acta. 1993 Oct 29;1154(2):157-72. doi: 10.1016/0304-4157(93)90009-d. Biochim Biophys Acta. 1993. PMID: 8218336 Review.
Cited by
-
Expression of Amino Acid Transporters (LAT1 and ASCT2) in Patients with Stage III/IV Laryngeal Squamous Cell Carcinoma.Pathol Oncol Res. 2015 Sep;21(4):1175-81. doi: 10.1007/s12253-015-9954-3. Epub 2015 May 30. Pathol Oncol Res. 2015. PMID: 26024742
-
Hartnup disorder: polymorphisms identified in the neutral amino acid transporter SLC1A5.J Inherit Metab Dis. 2002 Oct;25(6):437-48. doi: 10.1023/a:1021286714582. J Inherit Metab Dis. 2002. PMID: 12555937
-
Targeting Solute Carrier Transporters (SLCs) as a Therapeutic Target in Different Cancers.Diseases. 2024 Mar 21;12(3):63. doi: 10.3390/diseases12030063. Diseases. 2024. PMID: 38534987 Free PMC article. Review.
-
NDRG2 ablation reprograms metastatic cancer cells towards glutamine dependence via the induction of ASCT2.Int J Biol Sci. 2020 Oct 16;16(16):3100-3115. doi: 10.7150/ijbs.48066. eCollection 2020. Int J Biol Sci. 2020. PMID: 33162818 Free PMC article.
-
Transport of L-Arginine Related Cardiovascular Risk Markers.J Clin Med. 2020 Dec 8;9(12):3975. doi: 10.3390/jcm9123975. J Clin Med. 2020. PMID: 33302555 Free PMC article. Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
