doi: 10.1016/0378-1119(96)00059-5.
Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae
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- PMID: 8654982
- DOI: 10.1016/0378-1119(96)00059-5
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Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae
Gene.
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Abstract
We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.
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