Human papillomavirus type 16 E7 alleviates a proliferation block in early passage human mammary epithelial cells
- PMID: 8622898
Human papillomavirus type 16 E7 alleviates a proliferation block in early passage human mammary epithelial cells
Abstract
Human mammary epithelial cells (HMEC) isolated from reduction mammoplasty tissue are proliferative for several passages but then enter a period termed ¿selection' or M0 during which the majority of the cells become larger, flattened, and less proliferative. Early passage HMEC (prior to M0) were transduced with human papillomavirus type-16 E6, E7, or E6/E7 by using recombinant retroviral vectors. E7 alone or E6/E7, but not E6 alone, alleviated the M0 proliferation block, suggesting a possible role for Rb or Rb-related proteins, but not p53, in M0. In addition, cells in M0 did not have increased levels of p53 or p21 proteins, further indicating that induction of these proteins is not required for the M0 proliferation block. Early passage cells contained Rb which was primarily hyperphosphorylated while cells in M0 contained Rb protein which was predominantly underphosphorylated. Cells in M0 accumulated in G1 or B0 and expressed reduced levels of cyclin A and CDK2 proteins.
Similar articles
-
TGF-beta-mediated cell cycle arrest of HPV16-immortalized human ectocervical cells correlates with decreased E6/E7 mRNA and increased p53 and p21(WAF-1) expression.Exp Cell Res. 2000 Aug 25;259(1):149-57. doi: 10.1006/excr.2000.4953. Exp Cell Res. 2000. PMID: 10942587
-
Sequential extension of proliferative lifespan in human fibroblasts induced by over-expression of CDK4 or 6 and loss of p53 function.Oncogene. 2002 Jun 20;21(27):4277-88. doi: 10.1038/sj.onc.1205492. Oncogene. 2002. PMID: 12082615
-
p53-independent growth regulation of cervical cancer cells by the papillomavirus E6 oncogene.Oncogene. 1996 Sep 5;13(5):1027-35. Oncogene. 1996. PMID: 8806692
-
Differential patterns of cell cycle regulatory proteins expression in transgenic models of thyroid tumours.Oncogene. 1998 Aug 6;17(5):631-41. doi: 10.1038/sj.onc.1201966. Oncogene. 1998. PMID: 9704929
-
The cell cycle: accelerators, brakes, and checkpoints.Neurosurgery. 2004 Mar;54(3):692-9; discussion 699-700. doi: 10.1227/01.neu.0000109534.28063.5d. Neurosurgery. 2004. PMID: 15028146 Review.
Cited by
-
Cytogenetic characterization and H-ras associated transformation of immortalized human mammary epithelial cells.Cancer Cell Int. 2006 May 26;6:15. doi: 10.1186/1475-2867-6-15. Cancer Cell Int. 2006. PMID: 16729884 Free PMC article.
-
Genetic and epigenetic changes in mammary epithelial cells may mimic early events in carcinogenesis.J Mammary Gland Biol Neoplasia. 2004 Jul;9(3):263-74. doi: 10.1023/B:JOMG.0000048773.95897.5f. J Mammary Gland Biol Neoplasia. 2004. PMID: 15557799 Review.
-
Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT.Cancer Sci. 2007 Feb;98(2):147-54. doi: 10.1111/j.1349-7006.2006.00373.x. Cancer Sci. 2007. PMID: 17233832 Free PMC article.
-
Epigenetic Reprogramming of Lineage-Committed Human Mammary Epithelial Cells Requires DNMT3A and Loss of DOT1L.Stem Cell Reports. 2017 Sep 12;9(3):943-955. doi: 10.1016/j.stemcr.2017.06.019. Epub 2017 Aug 3. Stem Cell Reports. 2017. PMID: 28781076 Free PMC article.
-
Papillomavirus type 16 oncogenes downregulate expression of interferon-responsive genes and upregulate proliferation-associated and NF-kappaB-responsive genes in cervical keratinocytes.J Virol. 2001 May;75(9):4283-96. doi: 10.1128/JVI.75.9.4283-4296.2001. J Virol. 2001. PMID: 11287578 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Research Materials
Miscellaneous