The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity
- PMID: 8621531
- DOI: 10.1074/jbc.271.15.8887
The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity
Abstract
We have cloned and characterized the Hansenula polymorpha PER9 gene by functional complementation of the per9-1 mutant of H. polymorpha, which is defective in peroxisome biogenesis. The predicted product, Per9p, is a polypeptide of 52 kDa with sequence similarity to Pas3p, a protein involved in peroxisome biogenesis in Saccharomyces cerevisiae. In a per9 disruption strain (Deltaper9), peroxisomal matrix and membrane proteins are present at wild-type levels. The matrix proteins accumulated in the cytoplasm. However, the location of the membrane proteins remained obscure; fully induced Deltaper9 cells lacked residual peroxisomal vesicles ("ghosts"). Analysis of the activity of the PER9 promoter revealed that PER9 expression was low in cells grown on glucose, but was enhanced during growth of cells on peroxisome-inducing substrates. The highest expression levels were observed in cells grown on methanol. Localization studies revealed that Per9p is an integral membrane protein of the peroxisome. Targeting studies suggested that Per9p may be sorted to the peroxisome via the endoplasmic reticulum. Overexpression of PER9 induced a significant increase in the number of peroxisomes per cell, a result that suggests that Per9p may be involved in peroxisome proliferation and/or membrane biosynthesis. When PER9 expression was placed under the control of a strongly regulatable promoter and switched off, peroxisomes were observed to disintegrate over time in a manner that suggested that Per9p may be required for maintenance of the peroxisomal membrane.
Similar articles
-
Heterologous complementation of peroxisome function in yeast: the Saccharomyces cerevisiae PAS3 gene restores peroxisome biogenesis in a Hansenula polymorpha per9 disruption mutant.FEBS Lett. 1995 Dec 27;377(3):434-8. doi: 10.1016/0014-5793(95)01385-7. FEBS Lett. 1995. PMID: 8549771
-
Isolation and characterization of Pas2p, a peroxisomal membrane protein essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris.J Biol Chem. 1996 Aug 2;271(31):18973-80. doi: 10.1074/jbc.271.31.18973. J Biol Chem. 1996. PMID: 8702562
-
The Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis.EMBO J. 1997 Jan 2;16(1):44-53. doi: 10.1093/emboj/16.1.44. EMBO J. 1997. PMID: 9009266 Free PMC article.
-
Hansenula polymorpha: an attractive model organism for molecular studies of peroxisome biogenesis and function.FEMS Microbiol Lett. 1992 Dec 15;100(1-3):393-403. doi: 10.1111/j.1574-6968.1992.tb14068.x. FEMS Microbiol Lett. 1992. PMID: 1478473 Review.
-
Peroxisome biogenesis in Saccharomyces cerevisiae.Antonie Van Leeuwenhoek. 1992 Aug;62(1-2):63-78. doi: 10.1007/BF00584463. Antonie Van Leeuwenhoek. 1992. PMID: 1444337 Review.
Cited by
-
The peroxin pex3p initiates membrane assembly in peroxisome biogenesis.Mol Biol Cell. 2000 Jun;11(6):2085-102. doi: 10.1091/mbc.11.6.2085. Mol Biol Cell. 2000. PMID: 10848631 Free PMC article.
-
Peroxisomes: a nexus for lipid metabolism and cellular signaling.Cell Metab. 2014 Mar 4;19(3):380-92. doi: 10.1016/j.cmet.2014.01.002. Epub 2014 Feb 6. Cell Metab. 2014. PMID: 24508507 Free PMC article. Review.
-
Emerging role of the endoplasmic reticulum in peroxisome biogenesis.Front Physiol. 2013 Oct 8;4:286. doi: 10.3389/fphys.2013.00286. Front Physiol. 2013. PMID: 24115935 Free PMC article. Review.
-
Pex19p, a farnesylated protein essential for peroxisome biogenesis.Mol Cell Biol. 1998 Jan;18(1):616-28. doi: 10.1128/MCB.18.1.616. Mol Cell Biol. 1998. PMID: 9418908 Free PMC article.
-
The cytosolic and membrane components required for peroxisomal protein import.Experientia. 1996 Dec 15;52(12):1050-4. doi: 10.1007/BF01952101. Experientia. 1996. PMID: 8988245 Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases