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. 1996 Jan;42(1):30-6.
doi: 10.1007/BF00163208.

Flanking sequences of an Alu source stimulate transcription in vitro by interacting with sequence-specific transcription factors

I Chesnokov  1 , C W Schmid
Affiliations

Flanking sequences of an Alu source stimulate transcription in vitro by interacting with sequence-specific transcription factors

I Chesnokov et al. J Mol Evol. 1996 Jan.

Abstract

An Alu source gene, called the EPL Alu, was previously isolated by a phylogenetic strategy. Sequences flanking the EPL Alu family member stimulate its RNA polymerase III (Pol III) template activity in vitro. One cis-acting element maps within a 40-nucleotide region immediately upstream to the EPL Alu. This same region contains an Ap1 site which, when mutated, abolishes the transcriptional stimulation provided by this region. The flanking sequence, as assayed by gel mobility shift, forms sequence-specific complexes with several nuclear factors including Ap1. These results demonstrate that an an ancestral Alu source sequence fortuitously acquired positive transcriptional control elements by insertion into the EPL locus, thereby providing biochemical evidence for a model which explains the selective amplification of Alu subfamilies.

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References

    1. Mol Cell Biol. 1990 Oct;10(10):5424-32 - PubMed
    1. Trends Genet. 1992 Sep;8(9):307-11 - PubMed
    1. Nucleic Acids Res. 1990 Dec 11;18(23 ):6779-84 - PubMed
    1. Cell. 1989 Jun 2;57(5):825-34 - PubMed
    1. Curr Opin Genet Dev. 1992 Dec;2(6):874-82 - PubMed

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