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. 1996 Feb;16(2):468-74.
doi: 10.1128/MCB.16.2.468.

Repression of beta interferon gene expression in virus-infected cells is correlated with a poly(A) tail elongation

E Dehlin  1 , A von Gabain, G Alm, R Dingelmaier, O Resnekov
Affiliations

Repression of beta interferon gene expression in virus-infected cells is correlated with a poly(A) tail elongation

E Dehlin et al. Mol Cell Biol. 1996 Feb.

Abstract

Expression of beta interferon (IFN-beta) is transiently induced when Namalwa B cells (Burkitt lymphoma cell line) are infected by Sendai virus. In this study, we found that an elongation of the IFN-beta mRNA could be detected in virus-infected cells and that such a modification was not observed when the IFN-beta transcript was induced by a nonviral agent, poly(I-C). Treatment of the cells with a transcriptional inhibitor (actinomycin D or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) resulted in further elongation of the transcript. Characterization of the elongated IFN-beta transcript by primer extension and RNase H treatment showed that the modification was a result of an elongated poly(A) tail of up to 400 nucleotides. We conclude that the poly(A) tail elongation of the IFN-beta transcript is associated with the viral infection. Furthermore, the presence of the elongated IFN-beta transcript correlated with a decrease of IFN-beta protein in the medium and in cell extracts. Sucrose gradient analysis of cytoplasmic extracts showed that IFN-beta transcripts with elongated poly(A) tails were found in the nonpolysomal fractions, whereas the shorter transcripts could be detected in both polysomal and nonpolysomal fractions. A longer form of the IFN-beta mRNA was also found in the nonpolysomal fractions of cells not treated with transcriptional inhibitors. Thus, the observed regulation of IFN-beta mRNA is not entirely dependent on the inhibition of transcription. To our knowledge, this study provides the first example of a poly(A) tail elongation in somatic cells that negatively influences gene expression in vivo.

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References

    1. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7799-803 - PubMed
    1. Eur J Biochem. 1983 Jun 15;133(2):399-404 - PubMed
    1. Nucleic Acids Res. 1993 Jun 25;21(12):2917-20 - PubMed
    1. Nucleic Acids Res. 1979 Sep 11;7(1):179-92 - PubMed
    1. Genes Dev. 1987 Dec;1(10):1201-11 - PubMed

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