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. 1995 Aug;60(1):33-40.
doi: 10.1016/0304-3991(95)00093-g.

Under buffer SFM observation of immunospecies adsorbed on a cyano grafted silicon substrate

P E Mazeran  1 , J L Loubet, C Martelet, A Theretz
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Under buffer SFM observation of immunospecies adsorbed on a cyano grafted silicon substrate

P E Mazeran et al. Ultramicroscopy. 1995 Aug.

Abstract

Scanning force microscopy (SFM) in contact mode and in liquid medium has been employed to study immunospecies layers adsorbed on a silicon wafer. The silicon wafer has been grafted with a cyanosilane monolayer in order to create a surface with strong adhesive properties which prevent proteins being swept by the scan of the SFM tip. The force curves reveal that the adhesive force has been increased by a factor six without roughness modification (< 1 nm). After the incubation of the surface in a monoclonal antibody (mouse anti-human alpha-fetoprotein IgG) solution, SFM surface images suggest an homogeneous layer composed by ellipsoidal objects (40-60 nm in diameter, 6-13 nm in height). The substrate was moreover incubated in an antigenic solution (human alpha-fetoprotein): SFM images reveal that proteins have been added onto the antibody layer.

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