Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Nov 3;83(3):387-95.
doi: 10.1016/0092-8674(95)90116-7.

Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

J F McBlane  1 , D C van Gent, D A Ramsden, C Romeo, C A Cuomo, M Gellert, M A Oettinger
Affiliations
Free article

Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

J F McBlane et al. Cell. .
Free article

Abstract

Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5' end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5'-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources