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. 1993;3(1):77-82.

Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III

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Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III

N A Woychik et al. Gene Expr. 1993.

Abstract

The characterization of RNA polymerase subunit genes has revealed that some subunits are shared by the three nuclear enzymes, some are homologous, and some are unique to RNA polymerases I, II, or III. We report here the isolation and characterization of the yeast RNA polymerase II subunit RPB11, which is encoded by a single copy RPB11 gene located directly upstream of the topoisomerase I gene, TOPI, on chromosome XV. The sequence of the gene predicts an RPB11 subunit of 120 amino acids (13,600 daltons), only two amino acids shorter than the RPB9 polypeptide, that co-migrates with RPB11 under most SDS-PAGE conditions, RPB11 was found to be an essential gene that encodes a protein closely related to an essential subunit shared by RNA polymerases I and III, AC19. RPB11 contains a 19 amino acid segment found in three other yeast RNA polymerase subunits and the bacterial RNA polymerase subunit alpha. Some mutations that affect RNA polymerase assembly map within this segment, suggesting that this region may play a role in subunit interactions. As the isolation of RPB11 completes the isolation of known yeast RNA polymerase II subunit genes, we briefly summarize the salient features of these twelve genes and the polypeptides that they encode.

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Figures

Figure 1
Figure 1
The RPB11 gene. A. RPB11 is adjacent to the TOP1 gene. Arrows represent gene orientation; lengths of shaded and solid rectangles reflect relative gene sizes. B. Sequence of RPB11 DNA and the predicted amino acid sequence of the RPB11 subunit. The RPB11 peptides APNAVVITFEK, IQTTEGYDPK, LGALK, and TNFETEWNLQTLAA are underlined.
Figure 2
Figure 2
Copy number of the RPB11 gene. Genomic DNA prepared from haploid yeast cells was digested with the enzyme(s) indicated. DNA size markers (in kb) are shown to the right of each panel.
Figure 3
Figure 3
S. cerevisiae RPB11 is related to yeast RNA polymerase I and III subunit AC19 and contains a conserved 19 amino acid region present in the bacterial a subunit and other eukaryotic proteins related to α. A. Identical amino acid residues are boxed; conserved residues are in bold. B. RPB11 and RPB3, yeast RNA polymerase II subunits; AC19 and AC40, shared subunits of yeast RNA polymerase I and III; α, E. coli RNA polymerase subunit; RNLVA, α homologue in Marchantia polymorpha chloroplast DNA (Dequard-Chablat et al., 1991); cnjC, Tetrahymena conjugation-specific protein related to α, RPB3, and AC40 (Martindale, 1990). The shaded regions represent identical and conserved residues scoring +1 or greater on the Dayhoff mutation data matrix. The residues which contribute to a defect in RNA polymerase subunit assembly are marked with an asterisk. The RPB3 mutation A30 → D30 is represented in the mutant rpb3-1 (Kolodziej and Young, 1989). The α mutation R45 → C45 is represented in the mutant rpoA112 (Igarashi et al., 1990). Some of the sequence similarities were demonstrated in previous reports (Dequard-Chablat et al., 1991; Martindale, 1990).

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