doi: 10.1006/prep.1993.1022.
Overcoming inclusion body formation in a high-level expression system
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- PMID: 8471848
- DOI: 10.1006/prep.1993.1022
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Overcoming inclusion body formation in a high-level expression system
Protein Expr Purif.
1993 Apr.
Abstract
Attempts at overexpressing T4-phage deoxycytidylate deaminase using the pET3c/BL21(DE3)/pLysS system resulted in this enzyme being part of an inactive inclusion-body complex. However, by employing an enriched growth medium it was found that the deaminase could be induced in a soluble active form to at least 20% of this organism's cellular protein. Insoluble inclusion bodies were obtained with less rich media. This procedure was employed successfully with other highly expressed proteins that formed inclusion bodies. The use of a rich growth medium during the course of protein induction may be a valuable adjunct to limiting inclusion body formation with this as well as other expression systems.
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