Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Apr;31(4):904-10.
doi: 10.1128/jcm.31.4.904-910.1993.

Rapid, polymerase chain reaction-based identification assays for Candida species

H G Niesters  1 , W H Goessens, J F Meis, W G Quint
Affiliations

Rapid, polymerase chain reaction-based identification assays for Candida species

H G Niesters et al. J Clin Microbiol. 1993 Apr.

Abstract

Polymerase chain reaction (PCR) amplification of specific regions in the genomes of a variety of lower eukaryotes permits rapid identification of these microorganisms. First, on the basis of the presence of both constant and variable regions in the small subunit (ssu) rRNA, a nested PCR for direct identification of various Candida species can be designed. Amplification of the entire ssu rRNA gene and subsequent reamplification of variable sequences within the V4 domains of these PCR products were combined with direct sequencing. Restriction enzyme maps were made, and species-specific oligonucleotides for hybridization analysis were selected. Unequivocal discrimination of four of the major human pathogenic yeasts (Candida albicans, Candida glabrata, Candida tropicalis, and Candida krusei) is possible if a combination of these techniques is used. Second, by using oligonucleotides aimed at repeated sequences which occur at dispersed positions in the genomes of all eukaryotes, species-specific DNA fingerprints could be generated. This interrepeat PCR using genomic DNA as template proved to be an effective tool in Candida species typing. Both techniques described here can be extrapolated to the high-speed diagnostics of numerous other prokaryotic and eukaryotic pathogens.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nucleic Acids Res. 1989 Feb 11;17(3):1266 - PubMed
    1. J Med Vet Mycol. 1992;30(2):123-31 - PubMed
    1. Eur J Clin Microbiol Infect Dis. 1989 Oct;8(10):855-7 - PubMed
    1. Clin Microbiol Rev. 1990 Jan;3(1):32-45 - PubMed
    1. Hum Genet. 1990 Mar;84(4):301-36 - PubMed

LinkOut - more resources