Increased accumulation of drugs in a multidrug resistant cell line by alteration of membrane biophysical properties
- PMID: 8435444
- DOI: 10.1016/0167-4889(93)90217-d
Increased accumulation of drugs in a multidrug resistant cell line by alteration of membrane biophysical properties
Abstract
Growth of CHRC5 multidrug resistant cells in media enriched in a saturated C-17 fatty acid, heptadecanoic acid, resulted in these cells accumulating vinblastine at a rate and to an extent comparable to that of the parental cell line AB1. The fatty acid-enriched growth media had no effect on the ability of AB1 cells to take up vinblastine. The action of amphiphiles on the uptake of rhodamine dyes by CHRC5 cells was compared with the increased dye accumulation affected by verapamil. Membrane rigidifying agents, such as the saturated fatty acid stearic acid, or the cholesterol derivatives, cholesteryl hemisuccinate and cholesteryl phosphorylcholine, as well as a membrane fluidizing unsaturated fatty acid, linoleic acid, could significantly increase dye uptake, although not as well as verapamil. These results taken in conjunction with other reports in the literature, demonstrate that multidrug resistance is sensitive to alterations of membrane properties. They suggest that perturbation of the membrane to either increased or to decreased membrane fluidity can lower the level of resistance.
Similar articles
-
Solutol HS 15, nontoxic polyoxyethylene esters of 12-hydroxystearic acid, reverses multidrug resistance.Cancer Res. 1991 Feb 1;51(3):897-902. Cancer Res. 1991. PMID: 1988130
-
Resistance modulation in CHO cells by R-verapamil and bile salts is associated with physical and chemical changes in the cell membrane.Int J Clin Pharmacol Ther. 2000 Apr;38(4):196-203. doi: 10.5414/cpp38196. Int J Clin Pharmacol Ther. 2000. PMID: 10783829
-
The concomitant effect of unsaturated fatty acid supplemented to medium on cellular growth and membrane fluidity of cultured cells.Cell Biol Int Rep. 1979 Sep;3(6):515-23. doi: 10.1016/0309-1651(79)90087-0. Cell Biol Int Rep. 1979. PMID: 574063
-
Transport properties of P-glycoprotein in plasma membrane vesicles from multidrug-resistant Chinese hamster ovary cells.Biochim Biophys Acta. 1992 Aug 24;1109(2):161-71. doi: 10.1016/0005-2736(92)90079-2. Biochim Biophys Acta. 1992. PMID: 1355667
-
[Influence of linoleic acid (18:2 n-6) and alpha-linolenic acid (18:3 n-3) on the composition, permeability and fluidity of cardiac phospholipids in the rat: study using membrane models (liposomes)].Reprod Nutr Dev (1980). 1986;26(1A):97-112. Reprod Nutr Dev (1980). 1986. PMID: 2871601 French.
Cited by
-
Bioactive lipids in cancer stem cells.World J Stem Cells. 2019 Sep 26;11(9):693-704. doi: 10.4252/wjsc.v11.i9.693. World J Stem Cells. 2019. PMID: 31616544 Free PMC article. Review.
-
In vitro microfluidic models of tumor microenvironment to screen transport of drugs and nanoparticles.Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017 Sep;9(5):10.1002/wnan.1460. doi: 10.1002/wnan.1460. Epub 2017 Feb 14. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017. PMID: 28198106 Free PMC article. Review.
-
Ciprofloxacin is actively transported across bronchial lung epithelial cells using a Calu-3 air interface cell model.Antimicrob Agents Chemother. 2013 Jun;57(6):2535-40. doi: 10.1128/AAC.00306-13. Epub 2013 Mar 18. Antimicrob Agents Chemother. 2013. PMID: 23507281 Free PMC article.
-
Machine Learning Uncovers Food- and Excipient-Drug Interactions.Cell Rep. 2020 Mar 17;30(11):3710-3716.e4. doi: 10.1016/j.celrep.2020.02.094. Cell Rep. 2020. PMID: 32187543 Free PMC article.
-
Biophysics of cell membrane lipids in cancer drug resistance: Implications for drug transport and drug delivery with nanoparticles.Adv Drug Deliv Rev. 2013 Nov;65(13-14):1686-98. doi: 10.1016/j.addr.2013.09.004. Epub 2013 Sep 19. Adv Drug Deliv Rev. 2013. PMID: 24055719 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
