Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jan;52(1):46-59.

Identification of individuals by analysis of biallelic DNA markers, using PCR and solid-phase minisequencing

A C Syvänen  1 , A Sajantila, M Lukka
Affiliations

Identification of individuals by analysis of biallelic DNA markers, using PCR and solid-phase minisequencing

A C Syvänen et al. Am J Hum Genet. 1993 Jan.

Abstract

We have developed a new method for forensic identification of individuals, in which a panel of biallelic DNA markers are amplified by the PCR, and the variable nucleotides are detected in the amplified DNA fragments by the solid-phase minisequencing method. A panel of 12 common polymorphic nucleotides located on different chromosomes with reported allele frequencies close to .5 were chosen for the test. The allele frequencies for most of the markers were found to be similar in the Finnish and other Caucasian populations. We also introduce a novel approach for rapid determination of the population frequencies of biallelic markers. By this approach we were able to determine the allele frequencies of the markers in the Finnish population, by quantitative analysis of three pooled DNA samples representing 3,000 individuals. The power of discrimination and exclusion of the solid-phase minisequencing typing test with 12 markers was similar to that of three VNTR markers that are routinely used in forensic analyses at our institute. The solid-phase minisequencing method was successfully applied to type paternity and forensic case samples. We also show that the quantitative nature of our method allows typing of mixed samples.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Methods Enzymol. 1981;78(Pt A):29-38 - PubMed
    1. Nucleic Acids Res. 1991 Apr 25;19(8):1964 - PubMed
    1. Nature. 1985 Mar 7-13;314(6006):67-73 - PubMed
    1. Hum Hered. 1985;35(2):95-100 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5214-8 - PubMed

LinkOut - more resources