Genetic investigation of cAMP-dependent protein kinase function in Drosophila development
- PMID: 8391504
- DOI: 10.1101/gad.7.7a.1229
Genetic investigation of cAMP-dependent protein kinase function in Drosophila development
Abstract
The cAMP-dependent protein kinase (PKA) has been shown to mediate the vast majority of cellular responses to the intracellular second messenger, cAMP, in eukaryotes. To study the role of cAMP signal transduction in Drosophila development, we have isolated and molecularly characterized mutations of varying severity in the Drosophila PKA gene, DC0. Biochemical measurements indicate that DC0 is either the sole or the major PKA catalytic subunit gene in Drosophila. Adult females heterozygous for a strong and a weak DC0 allele fail to lay eggs and show a striking and novel defect in oogenesis that includes the formation of egg chambers containing multinucleate nurse cells. Females heterozygous for two weak DC0 alleles are fertile but produce offspring showing a variety of defects in embryogenesis, including preblastoderm arrest and alterations in cuticular patterning. Animals zygotically null for DC0 die as morphologically normal first-instar larvae, implying that maternally encoded protein, which perdures for at least 12 hr, suffices for embryogenesis. Animals hemizygous for weak DC0 alleles survive for several days as larvae but grow slowly. Mitotic recombination experiments in the adult eye indicate that the DC0 gene is not required autonomously either for cell viability or normal growth rates. These results argue that cAMP-mediated signal transduction is essential at a variety of stages during the development of a metazoan.
Similar articles
-
Gprk2 controls cAMP levels in Drosophila development.Dev Biol. 2001 May 1;233(1):174-85. doi: 10.1006/dbio.2001.0219. Dev Biol. 2001. PMID: 11319866
-
Activity, expression and function of a second Drosophila protein kinase A catalytic subunit gene.Genetics. 1995 Dec;141(4):1507-20. doi: 10.1093/genetics/141.4.1507. Genetics. 1995. PMID: 8601490 Free PMC article.
-
Localization and functions of protein kinase A during Drosophila oogenesis.Mech Dev. 1995 Feb;49(3):191-200. doi: 10.1016/0925-4773(94)00317-g. Mech Dev. 1995. PMID: 7734392
-
The ways in which hormones change cyclic adenosine 3',5'-monophosphate-dependent protein kinase subunits, and how such changes affect cell behavior.Endocr Rev. 1993 Oct;14(5):632-50. doi: 10.1210/edrv-14-5-632. Endocr Rev. 1993. PMID: 8262010 Review.
-
Serine/threonine protein kinases in Drosophila.Trends Genet. 1990 Nov;6(11):357-62. doi: 10.1016/0168-9525(90)90277-d. Trends Genet. 1990. PMID: 2150900 Review.
Cited by
-
Identification of new X-chromosomal genes required for Drosophila oogenesis and novel roles for fs(1)Yb, brainiac and dunce.Genome Res. 2001 Jan;11(1):67-77. doi: 10.1101/gr.156001. Genome Res. 2001. PMID: 11156616 Free PMC article.
-
Glial ensheathment of peripheral axons in Drosophila.J Neurosci Res. 2008 May 1;86(6):1189-98. doi: 10.1002/jnr.21574. J Neurosci Res. 2008. PMID: 18041093 Free PMC article. Review.
-
Postsynaptic cAMP signalling regulates the antagonistic balance of Drosophila glutamate receptor subtypes.Development. 2020 Dec 16;147(24):dev191874. doi: 10.1242/dev.191874. Development. 2020. PMID: 33234716 Free PMC article.
-
The contributions of protein kinase A and smoothened phosphorylation to hedgehog signal transduction in Drosophila melanogaster.Genetics. 2006 Aug;173(4):2049-62. doi: 10.1534/genetics.106.061036. Epub 2006 Jun 18. Genetics. 2006. PMID: 16783001 Free PMC article.
-
Mapping Second Chromosome Mutations to Defined Genomic Regions in Drosophila melanogaster.G3 (Bethesda). 2018 Jan 4;8(1):9-16. doi: 10.1534/g3.117.300289. G3 (Bethesda). 2018. PMID: 29066472 Free PMC article.
