Nucleotide sequence of the human coronavirus 229E RNA polymerase locus
- PMID: 8337838
- PMCID: PMC7131648
- DOI: 10.1006/viro.1993.1419
Nucleotide sequence of the human coronavirus 229E RNA polymerase locus
Abstract
The nucleotide sequence of the human coronavirus 229E (HCV 229E) RNA polymerase gene and the 5' region of the genome has been determined. The polymerase gene is comprised of two large open reading frames, ORF1a and ORF1b, that contain 4086 and 2687 codons, respectively. ORF1b overlaps ORF1a by 43 bases in the (-1) reading frame. The in vitro translation of SP6 transcripts which include HCV 229E sequences encompassing the ORF1a/ORF1b junction show that expression of ORF1b can be mediated by ribosomal frame-shifting. The predicted translation products of ORF1a (454,200 molecular weight) and ORF1a/1b (754,200 molecular weight) have been compared to the predicted RNA polymerase gene products of infectious bronchitis virus (IBV) and murine hepatitis virus (MHV) and conserved structural features and putative functional domains have been identified. This analysis completes the nucleotide sequence of the HCV 229E genome.
Similar articles
-
Complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus.Virology. 1995 Feb 1;206(2):817-22. doi: 10.1006/viro.1995.1004. Virology. 1995. PMID: 7856095 Free PMC article.
-
Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily.J Virol. 1991 Jun;65(6):2910-20. doi: 10.1128/JVI.65.6.2910-2920.1991. J Virol. 1991. PMID: 1851863 Free PMC article.
-
The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism.Nucleic Acids Res. 1990 Apr 11;18(7):1825-32. doi: 10.1093/nar/18.7.1825. Nucleic Acids Res. 1990. PMID: 2159623 Free PMC article.
-
Functional and genetic analysis of coronavirus replicase-transcriptase proteins.PLoS Pathog. 2005 Dec;1(4):e39. doi: 10.1371/journal.ppat.0010039. Epub 2005 Dec 9. PLoS Pathog. 2005. PMID: 16341254 Free PMC article. Review.
-
The complete nucleotide sequence of avian infectious bronchitis virus: analysis of the polymerase-coding region.Adv Exp Med Biol. 1987;218:15-29. doi: 10.1007/978-1-4684-1280-2_3. Adv Exp Med Biol. 1987. PMID: 2829522 Review. No abstract available.
Cited by
-
Contributions of Hyperactive Mutations in Mpro from SARS-CoV-2 to Drug Resistance.ACS Infect Dis. 2024 Apr 12;10(4):1174-1184. doi: 10.1021/acsinfecdis.3c00560. Epub 2024 Mar 12. ACS Infect Dis. 2024. PMID: 38472113
-
Systematic Analyses of the Resistance Potential of Drugs Targeting SARS-CoV-2 Main Protease.ACS Infect Dis. 2023 Jul 14;9(7):1372-1386. doi: 10.1021/acsinfecdis.3c00125. Epub 2023 Jun 30. ACS Infect Dis. 2023. PMID: 37390404 Free PMC article.
-
Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanisms.Elife. 2022 Jun 20;11:e77433. doi: 10.7554/eLife.77433. Elife. 2022. PMID: 35723575 Free PMC article.
-
Design, Synthesis and Evaluation of Fused Bicyclo[2.2.2]octene as a Potential Core Scaffold for the Non-Covalent Inhibitors of SARS-CoV-2 3CLpro Main Protease.Pharmaceuticals (Basel). 2022 Apr 27;15(5):539. doi: 10.3390/ph15050539. Pharmaceuticals (Basel). 2022. PMID: 35631364 Free PMC article.
-
Identifying Inhibitors of -1 Programmed Ribosomal Frameshifting in a Broad Spectrum of Coronaviruses.Viruses. 2022 Jan 18;14(2):177. doi: 10.3390/v14020177. Viruses. 2022. PMID: 35215770 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources