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. 1993 Jul 16;74(1):105-14.
doi: 10.1016/0092-8674(93)90298-5.

A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA

M Tian  1 , T Maniatis
Affiliations

A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA

M Tian et al. Cell. .

Abstract

Female-specific splicing of Drosophila doublesex (dsx) pre-mRNA is regulated by the products of the transformer (tra) and transformer 2 (tra2) genes. In this paper we show that Tra and Tra2 act by recruiting general splicing factors to a regulatory element located downstream of a female-specific 3' splice site. Remarkably, Tra, Tra2, and members of the serine/arginine-rich (SR) family of general splicing factors are sufficient to commit dsx pre-mRNA to female-specific splicing, and individual SR proteins differ significantly in their ability to participate in commitment complex formation. Characterization of the proteins associated with affinity-purified complex formed on dsx pre-mRNA reveals the presence of Tra, Tra2, SR proteins, and additional unidentified components. We conclude that Tra, Tra2, and SR proteins are essential components of a splicing enhancer complex.

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