Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study
- PMID: 833203
- PMCID: PMC2110997
- DOI: 10.1083/jcb.72.2.441
Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study
Abstract
When biochemical studies on the liver are interpreted, the cells of the sinusoidal area frequently receive little attention because, compared to hepatocytes, their contribution to subcellular fractions is assumed insignificant. A systematic stereological analysis of liver parenchyma was therefore performed in order to determine the distribution of organelles and membranes between hepatocytic and nonhepatocytic cells, namely endothelial, Kupffer, and fat-storing cells. The livers were fixed by vascular perfusion and the data were corrected for systematic errors dur to section thickness and compression. The extracellular space compartment includes the lumina of sinusoids (10.6%), the space of Disse (4.9%), and the bile canaliculi (0.4%). Hepatocytes constitute 78% of parenchymal volume; the nonhepatocytes account for 6.3% and consist of 2.8% endothelial cells, 2.1% Kupffer cells, and 1.4% fat-storing cells. The nonhepatocytes contribute 55% of the volume of lipid droplets in the liver, 43% of the lysosomes, and 1.2% of the mitochondria. Although the nonhepatocytes account for only 8% of the total surface area of parenchymal membranes, they contain 26.5% of all the plasma membranes, 32.4% of the lysosomal membranes, 15.1% of the Golgi apparatus 6.4% of the endoplasmic reticulum, and 2.4% of the mitochondrial membranes. The data demonstrate the extent to which nonhepatocytic organelles can potentially contaminate subcellular fractions used for biochemical studies. Particularly important for the interpretation of studies on lysosomes, plasma membrane, and Golgi apparatus is the finding that an appreciable part of these organelles may be derived from cell types other than hepatocytes.
Similar articles
-
Lysosomal degradation of cell organelles. I. Ultrastructural analysis of uptake and digestion of intravenously injected mitochondria by Kupffer cells.Lab Invest. 1975 Sep;33(3):239-51. Lab Invest. 1975. PMID: 1160345
-
Immunoelectron microscopic localization of mannose-terminal glucocerebrosidase in lysosomes of rat liver Kupffer cells.J Histochem Cytochem. 1995 Feb;43(2):149-58. doi: 10.1177/43.2.7822772. J Histochem Cytochem. 1995. PMID: 7822772
-
Kupffer cell structure in the juvenile Nile crocodile, Crocodylus niloticus.J Morphol. 2014 Jan;275(1):1-8. doi: 10.1002/jmor.20188. Epub 2013 Oct 21. J Morphol. 2014. PMID: 24142864
-
Cellular pathophysiology of hepatitis.Am J Clin Pathol. 1976 May;65(5 Suppl):828-47. Am J Clin Pathol. 1976. PMID: 802747 Review.
-
[Observations on the ultrastructure of the carp liver (Cyprinus carpio L.)].Z Mikrosk Anat Forsch. 1974;88(4):745-58. Z Mikrosk Anat Forsch. 1974. PMID: 4467573 German.
Cited by
-
Rat and mouse cardiomyocytes show subtle differences in creatine kinase expression and compartmentalization.PLoS One. 2023 Nov 27;18(11):e0294718. doi: 10.1371/journal.pone.0294718. eCollection 2023. PLoS One. 2023. PMID: 38011179 Free PMC article.
-
NADP-dependent dehydrogenases in rat liver parenchyma. I. Methodological studies on the qualitative histochemistry of G6PDH, 6PGDH, malic enzyme and ICDH.Histochemistry. 1978 Jul 12;56(3-4):283-98. doi: 10.1007/BF00495990. Histochemistry. 1978. PMID: 29020
-
Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy.Cell Rep. 2023 Dec 26;42(12):113529. doi: 10.1016/j.celrep.2023.113529. Epub 2023 Dec 6. Cell Rep. 2023. PMID: 38060380 Free PMC article.
-
Uptake of circulating hyaluronic acid by the rat liver. Cellular localization in situ.Cell Tissue Res. 1985;242(3):505-10. doi: 10.1007/BF00225415. Cell Tissue Res. 1985. PMID: 4075374
-
Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis.World J Gastroenterol. 2005 Sep 7;11(33):5095-102. doi: 10.3748/wjg.v11.i33.5095. World J Gastroenterol. 2005. PMID: 16127736 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources