Establishment and characterization of a human lung adenocarcinoma cell line (LC-2/ad) producing alpha 1-antitrypsin in vitro
- PMID: 8291444
- DOI: 10.1111/j.1440-1827.1993.tb03232.x
Establishment and characterization of a human lung adenocarcinoma cell line (LC-2/ad) producing alpha 1-antitrypsin in vitro
Abstract
A new human cell line, LC-2/ad was established from pleural effusion of pulmonary adenocarcinoma of a 51 year old Japanese female. The LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes as observed with phase-contrast microscopy. The modal chromosome number was 53-56. Plating efficiency and doubling time were 6.8% and 58 h, respectively (32th passage). Immunocytochemically, the cells were strongly positive for CEA and cytokeratins including cytokeratin no. 18 which is present in simple epithelia. Ultrastructurally, the cultured cells were characterized by well-formed junctional complexes and microvilli. Subcutaneous injection of 5 x 10(6) cells into a nude mouse resulted in tumor formation classified histologically as a moderately differentiated adenocarcinoma. This cell line produced at least two functionally active trypsin inhibitors together with several proteinases in vitro. The main inhibitor was purified partially from the serum-free conditioned medium and confirmed immunologically as human alpha 1-antitrypsin (AAT). Immunohistochemically, the xenografted tumor was also positive for AAT. The cell line LC-2/ad is useful for the study of tumor-derived serine proteinase inhibitors, in particular AAT.
Similar articles
-
A new human hepatocellular carcinoma cell line (KYN-1) with a transformation to adenocarcinoma.In Vitro Cell Dev Biol. 1986 Nov;22(11):637-46. doi: 10.1007/BF02623477. In Vitro Cell Dev Biol. 1986. PMID: 2430933
-
Establishment and characterization of a human renal carcinoma cell line MRT-1, with special reference to the production of serine proteinase inhibitors.Hum Cell. 1995 Mar;8(1):11-8. Hum Cell. 1995. PMID: 7669747
-
New human colorectal carcinoma cell lines that secrete proteinase inhibitors in vitro.Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(3):157-65. doi: 10.1007/BF02899077. Virchows Arch B Cell Pathol Incl Mol Pathol. 1989. PMID: 2570482
-
[Establishment and characterization of human cancer cell line (NOC-S) derived from a progressive lung cancer].Hum Cell. 1990 Jun;3(2):158-62. Hum Cell. 1990. PMID: 2085480 Japanese.
-
Biliary adenocarcinoma. Characterisation of three new human tumor cell lines.J Hepatol. 1985;1(6):579-96. doi: 10.1016/s0168-8278(85)80002-7. J Hepatol. 1985. PMID: 4056357
Cited by
-
Identification of a lung adenocarcinoma cell line with CCDC6-RET fusion gene and the effect of RET inhibitors in vitro and in vivo.Cancer Sci. 2013 Jul;104(7):896-903. doi: 10.1111/cas.12175. Epub 2013 May 12. Cancer Sci. 2013. PMID: 23578175 Free PMC article.
-
Identification and characterization of different subpopulations in a human lung adenocarcinoma cell line (A549).Pathol Oncol Res. 1999;5(3):197-204. doi: 10.1053/paor.1999.0212. Pathol Oncol Res. 1999. PMID: 10491017
-
Expression of the Wilms' tumor gene WT1 in solid tumors and its involvement in tumor cell growth.Jpn J Cancer Res. 1999 Feb;90(2):194-204. doi: 10.1111/j.1349-7006.1999.tb00733.x. Jpn J Cancer Res. 1999. PMID: 10189890 Free PMC article.
-
Hepatocyte growth factor activator inhibitor type 1 suppresses metastatic pulmonary colonization of pancreatic carcinoma cells.Cancer Sci. 2011 Feb;102(2):407-13. doi: 10.1111/j.1349-7006.2010.01808.x. Epub 2010 Dec 20. Cancer Sci. 2011. PMID: 21166957 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Medical
Research Materials
Miscellaneous