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. 1993 Dec 11;21(24):5570-6.
doi: 10.1093/nar/21.24.5570.

Specific transcriptional activation in vitro by the herpes simplex virus protein VP16

D N Arnosti  1 , C M Preston, M Hagmann, W Schaffner, R G Hope, G Laughlan, B F Luisi
Affiliations
Free PMC article

Specific transcriptional activation in vitro by the herpes simplex virus protein VP16

D N Arnosti et al. Nucleic Acids Res. .
Free PMC article

Abstract

The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcription system. Stimulation of transcription was dependent on the IE-specific element, TAATGARAT. In agreement with earlier observations from electrophoretic mobility shift assays, activation was not observed when Oct-2/POU, the DNA-binding domain from the Oct-2 protein, was substituted for Oct-1/POU. Single round transcription assays revealed that, together, VP16 and Oct-1/POU facilitate the assembly of pre-initiation complexes at target gene promoters.

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References

    1. J Biol Chem. 1987 Mar 15;262(8):3452-61 - PubMed
    1. Cell. 1993 Jul 16;74(1):115-25 - PubMed
    1. Cell. 1988 Feb 12;52(3):425-34 - PubMed
    1. Cell. 1988 Feb 12;52(3):435-45 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6347-51 - PubMed

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