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Comparative Study
. 1994 Jan;242(1):1-8.
doi: 10.1007/BF00277341.

Isolation and characterization of a maize gene encoding chalcone flavonone isomerase

Affiliations
Comparative Study

Isolation and characterization of a maize gene encoding chalcone flavonone isomerase

E Grotewold et al. Mol Gen Genet. 1994 Jan.

Abstract

We report here the first cloning of a chalcone flavonone isomerase gene (CHI) from maize. Northern blot experiments indicate that the maize CHI gene (ZmCHI1) is regulated in the pericarp by the P gene, a myb homologue. The ZmCHI1 gene encodes a 24.3 kDa product 55% and 58% identical to CHI-A and CHI-B from Petunia, respectively. This maize CHI1 gene has four exons and an intron-exon structure identical to the CHI-B gene of Petunia hybrida. RFLP mapping data indicate that some inbred lines contain two additional CHI-homologous sequences, suggesting an organization more complex than that found in Petunia or bean. The possibility that the additional CHI-homologous sequences are responsible for the lack of CHI mutants in maize will be discussed.

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