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Comparative Study
. 1993 Dec;67(12):7190-200.
doi: 10.1128/JVI.67.12.7190-7200.1993.

Specific binding of human immunodeficiency virus type 1 gag polyprotein and nucleocapsid protein to viral RNAs detected by RNA mobility shift assays

R D Berkowitz  1 , J Luban, S P Goff
Affiliations
Comparative Study

Specific binding of human immunodeficiency virus type 1 gag polyprotein and nucleocapsid protein to viral RNAs detected by RNA mobility shift assays

R D Berkowitz et al. J Virol. 1993 Dec.

Abstract

Packaging of retroviral genomic RNA during virion assembly is thought to be mediated by specific interactions between the gag polyprotein and RNA sequences (often termed the psi or E region) near the 5' end of the genome. For many retroviruses, including human immunodeficiency virus type 1 (HIV-1), the portions of the gag protein and the RNA that are required for this interaction remain poorly defined. We have used an RNA gel mobility shift assay to measure the in vitro binding of purified glutathione S-transferase-HIV-1 gag fusion proteins to RNA riboprobes. Both the complete gag polyprotein and the nucleocapsid (NC) protein alone were found to bind specifically to an HIV-1 riboprobe. Either Cys-His box of NC could be removed without eliminating specific binding to the psi riboprobe, but portions of gag containing only the MA and CA proteins without NC did not bind to RNA. There were at least two binding sites in HIV-1 genomic RNA that bound to the gag polyprotein: one entirely 5' to gag and one entirely within gag. The HIV-1 NC protein bound to riboprobes containing other retroviral psi sequences almost as well as to the HIV-1 psi riboprobe.

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References

    1. J Virol. 1978 Apr;26(1):54-60 - PubMed
    1. J Biol Chem. 1987 Apr 15;262(11):4961-7 - PubMed
    1. Nucleic Acids Res. 1979 Dec 11;7(7):2055-72 - PubMed
    1. J Virol. 1980 Nov;36(2):450-6 - PubMed
    1. J Gen Virol. 1981 Aug;55(Pt 2):379-91 - PubMed

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