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. 1993 Oct;23(1):179-83.
doi: 10.1007/BF00021429.

A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance

C M Hsu  1 , W P Yang, C C Chen, Y K Lai, T Y Lin
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A point mutation in the chloroplast rps12 gene from Nicotiana plumbaginifolia confers streptomycin resistance

C M Hsu et al. Plant Mol Biol. 1993 Oct.

Abstract

In an effort to understand the mechanism of streptomycin resistance in Nicotiana plumbaginifolia, we have sequenced the chloroplast rps12 gene, a potential molecular target. We report that a streptomycin-resistant mutant isolated from protoplast cultures of N. plumbaginifolia contains an A-to-G transition at nucleotide position 149 in exon 2 of the chloroplast rps12 gene. The detected point mutation predicts a substitution of arginine for lysine in a phylogenetically conserved region.

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