Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71
- PMID: 8213
- DOI: 10.1016/0092-8674(76)90148-3
Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71
Abstract
The 71,000 dalton glycoprotein (gp71) purified from Rauscher murine leukemia virus (R-MuLV) by affinity chromatography specifically binds to murine but not other mammalian cells in culture. Binding is prevented by specific antiserum raides to gp71 (anti-gp71). The binding assay as described in this report can detect receptors on as few as 300 murine cells, and with 1 X 10(5) cells gives significant binding with 30 sec. The results show that the purified glycoprotein retians biologic activity and can form a stable complex with specific receptors on mouse cell membranes. The assay can therefore be used to characterize the nature of the cellular receptors that are essential for leukemia virus infection. Purified gp71 binding to mouse cells is prevented if the cells are actively producing related ecotropic type C viruses, presumably because the receptors are occupied and are not available to bind exogenously applied gp71. The binding of gp71 to murine cells is enhanced by the presence of calcium ions and low pH. Binding studies performed using an excess of 125I-gp71 indicate the NIH/3T3 cells bind approximately 5.3 X 10(5) molecules of 125I-gp71 per cell.
Similar articles
-
Binding characteristics of Rauscher leukemia virus envelope glycoprotein gp71 to murine lymphoid cells.J Virol. 1977 Dec;24(3):729-35. doi: 10.1128/JVI.24.3.729-735.1977. J Virol. 1977. PMID: 563478 Free PMC article.
-
A rapid bioassay to monitor murine leukemia virus infection in mice using cellular gp 71 binding.J Virol Methods. 1982 May;4(4-5):209-17. doi: 10.1016/0166-0934(82)90067-2. J Virol Methods. 1982. PMID: 6286701
-
Depression of Rauscher leukemia virus envelope glycoprotein gp71 binding by lymphoid cells during leukemogenesis in mice.Infect Immun. 1979 Jun;24(3):647-55. doi: 10.1128/iai.24.3.647-655.1979. Infect Immun. 1979. PMID: 468372 Free PMC article.
-
Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice.J Virol. 1976 Mar;17(3):727-36. doi: 10.1128/JVI.17.3.727-736.1976. J Virol. 1976. PMID: 56459 Free PMC article.
-
Cell surface receptors for ecotropic MuLV: detection and tissue distributions of free receptors in vivo.Int J Cancer. 1978 Mar 15;21(3):356-60. doi: 10.1002/ijc.2910210317. Int J Cancer. 1978. PMID: 631935
Cited by
-
TGF-β-Based Therapies for Treating Ocular Surface Disorders.Cells. 2024 Jun 26;13(13):1105. doi: 10.3390/cells13131105. Cells. 2024. PMID: 38994958 Free PMC article. Review.
-
Carbohydrate side chains of Rauscher leukemia virus envelope glycoproteins are not required to elicit a neutralizing antibody response.J Virol. 1986 Jan;57(1):340-2. doi: 10.1128/JVI.57.1.340-342.1986. J Virol. 1986. PMID: 2416953 Free PMC article.
-
Binding characteristics of Rauscher leukemia virus envelope glycoprotein gp71 to murine lymphoid cells.J Virol. 1977 Dec;24(3):729-35. doi: 10.1128/JVI.24.3.729-735.1977. J Virol. 1977. PMID: 563478 Free PMC article.
-
Genomic diversity of the acquired immunodeficiency syndrome retroviruses is reflected in alteration of its translational products.Proc Natl Acad Sci U S A. 1986 Aug;83(15):5718-22. doi: 10.1073/pnas.83.15.5718. Proc Natl Acad Sci U S A. 1986. PMID: 3016717 Free PMC article.
-
Nucleotide sequence of the envelope gene of Friend murine leukemia virus.J Virol. 1983 Jan;45(1):1-9. doi: 10.1128/JVI.45.1.1-9.1983. J Virol. 1983. PMID: 6296423 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
