Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins
- PMID: 8202553
- PMCID: PMC44067
- DOI: 10.1073/pnas.91.12.5715
Neurally expressed Drosophila genes encoding homologs of the NSF and SNAP secretory proteins
Abstract
Several lines of investigation have now converged to indicate that the neurotransmitter release apparatus is formed by assembly of cytosolic proteins with proteins of the synaptic vesicle and presynaptic terminal membranes. We are undertaking a genetic approach in Drosophila melanogaster to investigate the functions of two types of cytosolic proteins thought to function in this complex: N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs). We have identified Drosophila homologs of the vertebrate and yeast NSF and SNAP genes. Both Drosophila genes encode polypeptides that closely resemble their vertebrate counterparts and are expressed in the nervous system; neither appears to be in a family of closely related Drosophila genes. These results indicate that the Drosophila NSF and SNAP genes are excellent candidates for mutational analysis of neurotransmitter release.
Similar articles
-
Genetic analysis of soluble N-ethylmaleimide-sensitive factor attachment protein function in Drosophila reveals positive and negative secretory roles.J Neurosci. 2004 Apr 21;24(16):3964-73. doi: 10.1523/JNEUROSCI.5259-03.2004. J Neurosci. 2004. PMID: 15102912 Free PMC article.
-
The N-ethylmaleimide-sensitive fusion protein (NSF) is preferentially expressed in the nervous system.FEBS Lett. 1994 Jun 20;347(1):55-8. doi: 10.1016/0014-5793(94)00505-2. FEBS Lett. 1994. PMID: 8013662
-
Identification of a second homolog of N-ethylmaleimide-sensitive fusion protein that is expressed in the nervous system and secretory tissues of Drosophila.Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):7095-9. doi: 10.1073/pnas.92.15.7095. Proc Natl Acad Sci U S A. 1995. PMID: 7624376 Free PMC article.
-
Presynaptic proteins involved in exocytosis in Drosophila melanogaster: a genetic analysis.Invert Neurosci. 1995;1(1):3-13. doi: 10.1007/BF02331827. Invert Neurosci. 1995. PMID: 9372128 Review.
-
Molecular biology of ionotropic glutamate receptors in Drosophila melanogaster.Trends Pharmacol Sci. 1993 Dec;14(12):428-31. doi: 10.1016/0165-6147(93)90177-l. Trends Pharmacol Sci. 1993. PMID: 8122317 Review. No abstract available.
Cited by
-
The dominant cold-sensitive Out-cold mutants of Drosophila melanogaster have novel missense mutations in the voltage-gated sodium channel gene paralytic.Genetics. 2008 Oct;180(2):873-84. doi: 10.1534/genetics.108.090951. Epub 2008 Aug 24. Genetics. 2008. PMID: 18723887 Free PMC article.
-
Insight into Notch Signaling Steps That Involve pecanex from Dominant-Modifier Screens in Drosophila.Genetics. 2018 Aug;209(4):1099-1119. doi: 10.1534/genetics.118.300935. Epub 2018 May 31. Genetics. 2018. PMID: 29853475 Free PMC article.
-
Genetic modifiers of the Drosophila NSF mutant, comatose, include a temperature-sensitive paralytic allele of the calcium channel alpha1-subunit gene, cacophony.Genetics. 2000 May;155(1):203-11. doi: 10.1093/genetics/155.1.203. Genetics. 2000. PMID: 10790395 Free PMC article.
-
Huntingtin-interacting protein 14, a palmitoyl transferase required for exocytosis and targeting of CSP to synaptic vesicles.J Cell Biol. 2007 Dec 31;179(7):1481-96. doi: 10.1083/jcb.200710061. Epub 2007 Dec 24. J Cell Biol. 2007. PMID: 18158335 Free PMC article.
-
Genetic analysis of soluble N-ethylmaleimide-sensitive factor attachment protein function in Drosophila reveals positive and negative secretory roles.J Neurosci. 2004 Apr 21;24(16):3964-73. doi: 10.1523/JNEUROSCI.5259-03.2004. J Neurosci. 2004. PMID: 15102912 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
