Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1994 May 15;13(10):2464-71.
doi: 10.1002/j.1460-2075.1994.tb06531.x.

Many of the conserved nucleotides of tRNA(Phe) are not essential for ternary complex formation and peptide elongation

I A Nazarenko  1 , K M Harrington, O C Uhlenbeck
Affiliations
Comparative Study

Many of the conserved nucleotides of tRNA(Phe) are not essential for ternary complex formation and peptide elongation

I A Nazarenko et al. EMBO J. .

Abstract

An RNase protection assay was used to show that the dissociation rate constants and equilibrium constants of unmodified yeast and Escherichia coli phenylalanyl-tRNA(Phes) to elongation factor Tu from E.coli were very similar to each other and to their fully modified counterparts. The affinity of aminoacylated tRNA to elongation factor Tu was substantially lower when GTP analogues were used in place of GTP, emphasizing the importance of the beta-gamma phosphate linkage in the function of G-proteins. Fourteen different mutations in conserved and semi-conserved nucleotides of yeast phenylalanyl-tRNA(Phe) were tested for binding to elongation factor Tu.GTP and assayed for activity in the ribosomal A- and P-sites. Most of the mutations did not severely impair the function of these tRNAs in any of the assays. This suggests that the translational machinery does not form sequence-specific interactions with the conserved nucleotides of tRNA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nucleic Acids Res. 1991 Apr 25;19(8):1909-15 - PubMed
    1. Nucleic Acids Res. 1991 Feb 25;19(4):885-91 - PubMed
    1. Nucleic Acids Res. 1993 Feb 11;21(3):413-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Dec;84(24):8859-63 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 May 1;88(9):3725-8 - PubMed

Publication types

Substances