Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Apr 26;91(9):3862-6.
doi: 10.1073/pnas.91.9.3862.

Cellular latency in human immunodeficiency virus-infected individuals with high CD4 levels can be detected by the presence of promoter-proximal transcripts

M Adams  1 , L Sharmeen, J Kimpton, J M Romeo, J V Garcia, B M Peterlin, M Groudine, M Emerman
Affiliations

Cellular latency in human immunodeficiency virus-infected individuals with high CD4 levels can be detected by the presence of promoter-proximal transcripts

M Adams et al. Proc Natl Acad Sci U S A. .

Abstract

We have investigated the molecular basis of human immunodeficiency virus type 1 (HIV-1) latency in a tissue culture model and in HIV-infected people. We show that increased levels of Tat, but not Rev, can release the proviruses from latency in U1 cells. The absence of Tat in these cells is manifested by the accumulation of promoter-proximal viral transcripts, whereas the presence of Tat correlates with increased expression of viral proteins and an increase in promoter-distal transcripts. The presence of promoter-proximal transcripts also serves as a marker for latency in humans. We observed the exclusive presence of promoter-proximal viral transcripts in peripheral mononuclear cells from the majority (10/11) of asymptomatic HIV-infected individuals examined. Activation of these cells in vitro, and viremia in vivo, correlated with a switch from promoter-proximal transcription to promoter-distal transcription. These results suggest that the control between latency and replication of HIV in vivo is at the level of transcription elongation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1991 Oct 18;254(5030):423-7 - PubMed
    1. J Virol. 1992 Jan;66(1):310-6 - PubMed
    1. EMBO J. 1991 Dec;10(13):4189-96 - PubMed
    1. AIDS Res Hum Retroviruses. 1992 Feb;8(2):191-7 - PubMed
    1. AIDS. 1992 Jan;6(1):3-16 - PubMed

Publication types

MeSH terms