Type I phosphatidylinositol 4-phosphate 5-kinase isoforms are specifically stimulated by phosphatidic acid
- PMID: 8157686
Type I phosphatidylinositol 4-phosphate 5-kinase isoforms are specifically stimulated by phosphatidic acid
Abstract
A phosphatidylinositol 4-phosphate (PIP) kinase was isolated and purified to near homogeneity from bovine erythrocyte membranes. The PIP kinase was extracted from bovine erythrocyte membranes with a high salt wash, followed by phosphocellulose and phenyl-Sepharose chromatography. The predominant protein after phenyl-Sepharose purification had a molecular size of 68 kDa. Renaturation of PIP kinase activity after SDS-PAGE showed that a 68-kDa protein was able to phosphorylate PIP. An antibody developed against the 68-kDa protein Western blots the 68-kDa protein and is able to immunoprecipitate the 68-kDa protein and PIP kinase activity from membrane extracts. Based on functional studies, the 68-kDa protein is indistinguishable from the type I PIP kinase previously characterized from human erythrocyte membranes (Bazenet, C. E., Ruano, A.R., Brockman, J.L., and Anderson, R.A. (1990) J. Biol. Chem. 265, 18012-18022). These studies also show that the type I PIP kinases, but not the type II PIP kinase, are stimulated by phosphatidic acid, suggesting alternative roles for these enzymes. Two immunoreactive isoforms of the type I PIP kinase, of 68 and 90 kDa, were identified in rat brain and partially purified. Both of these isoforms are also stimulated by phosphatidic acid.
Similar articles
-
The human erythrocyte contains two forms of phosphatidylinositol-4-phosphate 5-kinase which are differentially active toward membranes.J Biol Chem. 1990 Oct 15;265(29):18012-22. J Biol Chem. 1990. PMID: 2170402
-
Purification and characterization of phosphatidylinositol 4-phosphate 5-kinases.Biochem J. 1992 Dec 1;288 ( Pt 2)(Pt 2):637-42. doi: 10.1042/bj2880637. Biochem J. 1992. PMID: 1334412 Free PMC article.
-
Partial purification and characterization of two forms of phosphatidylinositol 4-phosphate 5-kinase from human platelet membrane.Thromb Res. 1991 Oct 1;64(1):45-56. doi: 10.1016/0049-3848(91)90204-a. Thromb Res. 1991. PMID: 1663666
-
The phosphatidylinositol 4-phosphate 5-kinase family.Adv Enzyme Regul. 1996;36:115-40. doi: 10.1016/0065-2571(95)00005-4. Adv Enzyme Regul. 1996. PMID: 8869744 Review.
-
Phosphatidic acid regulation of phosphatidylinositol 4-phosphate 5-kinases.Biochim Biophys Acta. 2009 Sep;1791(9):905-12. doi: 10.1016/j.bbalip.2009.03.007. Epub 2009 Mar 17. Biochim Biophys Acta. 2009. PMID: 19298865 Review.
Cited by
-
Elicitation of suspension-cultured tomato cells triggers the formation of phosphatidic acid and diacylglycerol pyrophosphate.Plant Physiol. 2000 Aug;123(4):1507-16. doi: 10.1104/pp.123.4.1507. Plant Physiol. 2000. PMID: 10938366 Free PMC article.
-
Diacylglycerol kinases in immune cell function and self-tolerance.Immunol Rev. 2008 Aug;224:249-64. doi: 10.1111/j.1600-065X.2008.00647.x. Immunol Rev. 2008. PMID: 18759932 Free PMC article. Review.
-
Diacylglycerol kinase ζ generates dipalmitoyl-phosphatidic acid species during neuroblastoma cell differentiation.Biochem Biophys Rep. 2016 Oct 15;8:352-359. doi: 10.1016/j.bbrep.2016.10.004. eCollection 2016 Dec. Biochem Biophys Rep. 2016. PMID: 28955976 Free PMC article.
-
The phosphatidylinositol-transfer protein Nir2 binds phosphatidic acid and positively regulates phosphoinositide signalling.EMBO Rep. 2013 Oct;14(10):891-9. doi: 10.1038/embor.2013.113. Epub 2013 Jul 30. EMBO Rep. 2013. PMID: 23897088 Free PMC article.
-
PLD1 Negatively Regulates Dendritic Branching.J Neurosci. 2012 Jun 6;32(23):7960-9. doi: 10.1523/JNEUROSCI.5378-11.2012. J Neurosci. 2012. PMID: 22674271 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
