Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1994 Apr;125(1):31-42.
doi: 10.1083/jcb.125.1.31.

POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope

R W Wozniak  1 , G Blobel, M P Rout
Affiliations
Comparative Study

POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope

R W Wozniak et al. J Cell Biol. 1994 Apr.

Abstract

We have identified a concanavalin A-reactive glycoprotein of 150 kD that coenriches with isolated yeast nuclear pore complexes. Molecular cloning and sequencing of this protein revealed a single canonical transmembrane segment. Epitope tagging and localization by both immunofluorescence and immunoelectron microscopy confirmed that it is a pore membrane protein. The protein was termed POM152 (for pore membrane protein of 152 kD) on the basis of its location and cDNA-deduced molecular mass. POM152 is likely to be a type II membrane protein with its NH2-terminal region (175 residues) and its COOH-terminal region (1,142 residues) positioned on the pore side and cisternal side of the pore membrane, respectively. The proposed cisternally exposed domain contains eight repetitive motifs of approximately 24 residues. Surprisingly, POM152 deletion mutants were viable and their growth rate was indistinguishable from that of wild-type cells at temperatures between 17 and 37 degrees C. However, overproduction of POM152 inhibited cell growth. When expressed in mouse 3T3 cells, POM152 was found to be localized to the pore membrane, suggesting a conserved sorting pathway between yeast and mammals.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Int Rev Cytol Suppl. 1977;(6):75-186 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Feb;83(3):581-5 - PubMed
    1. Cell. 1987 May 8;49(3):295-7 - PubMed
    1. Nucleic Acids Res. 1987 Apr 24;15(8):3581-93 - PubMed
    1. Methods Enzymol. 1987;152:556-62 - PubMed

Publication types

MeSH terms

Associated data