doi: 10.1126/science.8128222.
Recombination between viral RNA and transgenic plant transcripts
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- PMID: 8128222
- DOI: 10.1126/science.8128222
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Recombination between viral RNA and transgenic plant transcripts
Science.
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Abstract
Transformed plants expressing the 3' two-thirds of the cowpea chlorotic mottle virus (CCMV) capsid gene were inoculated with a CCMV deletion mutant lacking the 3' one-third of the capsid gene. Although the deletion inoculum replicates in inoculated cells, systemic infections occur only if recombination restores a functional capsid gene. Four of 125 inoculated transgenic plants, representing three different transgenic lines, became systemically infected. Analysis of viral RNA confirmed that RNA recombination had united the transgenic messenger RNA and the challenging virus through aberrant homologous recombination.
Comment in
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Will transgenic crops generate new viruses and new diseases?Science. 1994 Mar 11;263(5152):1395-6. doi: 10.1126/science.8179685. Science. 1994. PMID: 8179685 No abstract available.
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Risks in using transgenic plants?Science. 1994 Jun 17;264(5166):1649-50; author reply 1651-2. doi: 10.1126/science.8209239. Science. 1994. PMID: 8209239 No abstract available.
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