Vascular endothelial growth factor is induced in response to transforming growth factor-beta in fibroblastic and epithelial cells
- PMID: 8119973
Vascular endothelial growth factor is induced in response to transforming growth factor-beta in fibroblastic and epithelial cells
Abstract
Transforming growth factor beta (TGF-beta) is a multifunctional polypeptide that regulates the proliferation and differentiation of various cells and has an angiogenic effect in vivo, although it inhibits the growth of cultured endothelial cells. We report here that TGF-beta treatment of quiescent cultures of mouse embryo-derived AKR-2B cells, which are growth-stimulated by TGF-beta, and human lung adenocarcinoma A549 cells, which are growth-inhibited by TGF-beta, results in the induction of vascular endothelial growth factor (VEGF) mRNA and protein. Maximal VEGF mRNA levels occurred 4-8 h after stimulation with a decline to background levels in 24 h. In contrast, the related placenta growth factor mRNA was not induced by TGF-beta in these cells. No VEGF receptor mRNA was seen in AKR-2B cells. Also, TGF-beta treatment of endothelial cells, which express the FLT1 and KDR/FLK-1 receptors for VEGF, did not cause VEGF induction. Because VEGF is known to be a strong angiogenic factor for endothelial cells, the results suggest that the angiogenic effect of TGF-beta on endothelial cells in blood vessels may be mediated at least partly by a paracrine induction of VEGF in other surrounding cell types.
Similar articles
-
Transforming growth factor-beta1 and ultraviolet A1 radiation increase production of vascular endothelial growth factor but not endothelin-1 in human dermal fibroblasts.Br J Dermatol. 2000 Sep;143(3):539-45. doi: 10.1111/j.1365-2133.2000.03707.x. Br J Dermatol. 2000. PMID: 10971326
-
Transforming growth factor-beta and Ras regulate the VEGF/VEGF-receptor system during tumor angiogenesis.Int J Cancer. 2002 Jan 10;97(2):142-8. doi: 10.1002/ijc.1599. Int J Cancer. 2002. PMID: 11774256
-
Purification and characterization of a naturally occurring vascular endothelial growth factor.placenta growth factor heterodimer.J Biol Chem. 1995 Mar 31;270(13):7717-23. doi: 10.1074/jbc.270.13.7717. J Biol Chem. 1995. PMID: 7706320
-
Expression of multiple angiogenic cytokines in cultured normal human prostate epithelial cells: predominance of vascular endothelial growth factor.Int J Cancer. 1999 Mar 15;80(6):868-74. doi: 10.1002/(sici)1097-0215(19990315)80:6<868::aid-ijc12>3.0.co;2-1. Int J Cancer. 1999. PMID: 10074920
-
Platelet factor-4 inhibits the mitogenic activity of VEGF121 and VEGF165 using several concurrent mechanisms.J Biol Chem. 1995 Jun 23;270(25):15059-65. doi: 10.1074/jbc.270.25.15059. J Biol Chem. 1995. PMID: 7797488
Cited by
-
Circulating proangiogenic cytokines and angiogenesis inhibitor endostatin in untreated patients with chronic lymphocytic leukemia.Mediators Inflamm. 2003 Jun;12(3):167-71. doi: 10.1080/0962935031000134888. Mediators Inflamm. 2003. PMID: 12857600 Free PMC article.
-
Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis.Biol Reprod. 2010 Jul;83(1):27-35. doi: 10.1095/biolreprod.109.082412. Epub 2010 Mar 24. Biol Reprod. 2010. PMID: 20335639 Free PMC article.
-
Nanotechnology in metastatic cancer treatment: Current Achievements and Future Research Trends.J Cancer. 2019 Feb 7;10(6):1358-1369. doi: 10.7150/jca.28394. eCollection 2019. J Cancer. 2019. PMID: 31031845 Free PMC article. Review.
-
Endothelial receptor tyrosine kinases involved in angiogenesis.J Cell Biol. 1995 May;129(4):895-8. doi: 10.1083/jcb.129.4.895. J Cell Biol. 1995. PMID: 7538139 Free PMC article. Review. No abstract available.
-
Cytokines in tumour growth, migration and metastasis.World J Urol. 1996;14(3):157-65. doi: 10.1007/BF00186895. World J Urol. 1996. PMID: 8806194 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
