cDNA cloning of an alternatively spliced isoform of the regulatory subunit of Ca2+/calmodulin-dependent protein phosphatase (calcineurin B alpha 2)
- PMID: 8110831
- DOI: 10.1016/0167-4781(94)90031-0
cDNA cloning of an alternatively spliced isoform of the regulatory subunit of Ca2+/calmodulin-dependent protein phosphatase (calcineurin B alpha 2)
Abstract
A cDNA encoding the regulatory subunit of Ca2+/calmodulin-dependent protein phosphatase, calcineurin B (CNB), was isolated from a rat testis cDNA library. It differs from the cDNA obtained from a rat brain cDNA library by an addition of 138 base pairs in the coding region. The codon of the clone from a testis library corresponding to the initiation codon of the clone from a brain library is not ATG but AAG, 5'-noncoding regions of these cDNAs are also different. The addition in the coding region results in the gain of 46 amino acids at the N-terminus. These findings suggest that two distinct isoforms of CNB alpha are derived from the same gene through a process involving alternative utilization of two promoters. We designate the brain type isoform as CNB alpha 1 and the longer isoform as CNB alpha 2. Northern blot analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) followed by Southern blot analysis suggest that CNB alpha 2 is specifically expressed in the testis, and its expression is developmentally regulated.
Similar articles
-
Isolation and sequence of rat testis cDNA for a calcium binding polypeptide similar to the regulatory subunit of calcineurin.Biochem Biophys Res Commun. 1991 Nov 14;180(3):1476-82. doi: 10.1016/s0006-291x(05)81362-2. Biochem Biophys Res Commun. 1991. PMID: 1659420
-
Cloning and characterization of molecular isoforms of the catalytic subunit of calcineurin using nonisotopic methods.J Biol Chem. 1990 Jul 5;265(19):11312-9. J Biol Chem. 1990. PMID: 2162844
-
Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).Biochem Biophys Res Commun. 1992 Aug 31;187(1):537-43. doi: 10.1016/s0006-291x(05)81527-x. Biochem Biophys Res Commun. 1992. PMID: 1325794
-
Identification of a third alternatively spliced cDNA encoding the catalytic subunit of protein phosphatase 2B beta.Biochim Biophys Acta. 1991 Feb 16;1088(2):308-10. doi: 10.1016/0167-4781(91)90069-x. Biochim Biophys Acta. 1991. PMID: 1848109
-
Calcineurin: not just a simple protein phosphatase.Biochem Biophys Res Commun. 1997 Jun 18;235(2):271-5. doi: 10.1006/bbrc.1997.6802. Biochem Biophys Res Commun. 1997. PMID: 9199180 Review.
Cited by
-
Serine/threonine protein phosphatases.Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):17-29. doi: 10.1042/bj3110017. Biochem J. 1995. PMID: 7575450 Free PMC article. Review. No abstract available.
-
The Impact of Electrographic Seizures on Developing Hippocampal Dendrites Is Calcineurin Dependent.eNeuro. 2017 Apr 28;4(2):ENEURO.0014-17.2017. doi: 10.1523/ENEURO.0014-17.2017. eCollection 2017 Mar-Apr. eNeuro. 2017. PMID: 28462391 Free PMC article.
-
Characterization of a human regulatory subunit of protein phosphatase 3 gene (PPP3RL) expressed specifically in testis.Mol Biol Rep. 2005 Mar;32(1):41-5. doi: 10.1007/s11033-004-4250-4. Mol Biol Rep. 2005. PMID: 15865209
-
Activity Regulates Cell Death within Cortical Interneurons through a Calcineurin-Dependent Mechanism.Cell Rep. 2018 Feb 13;22(7):1695-1709. doi: 10.1016/j.celrep.2018.01.007. Cell Rep. 2018. PMID: 29444424 Free PMC article.
-
Potential role of calcineurin in pathogenic conditions.Mol Cell Biochem. 2010 May;338(1-2):133-41. doi: 10.1007/s11010-009-0346-4. Epub 2009 Dec 5. Mol Cell Biochem. 2010. PMID: 19967549 Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
