Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Jul 1;269(26):17401-10.

An amino acid sequence shared by the herpes simplex virus 1 alpha regulatory proteins 0, 4, 22, and 27 predicts the nucleotidylylation of the UL21, UL31, UL47, and UL49 gene products

J A Blaho  1 , C Mitchell, B Roizman
Affiliations
  • PMID: 8021242
Free article

An amino acid sequence shared by the herpes simplex virus 1 alpha regulatory proteins 0, 4, 22, and 27 predicts the nucleotidylylation of the UL21, UL31, UL47, and UL49 gene products

J A Blaho et al. J Biol Chem. .
Free article

Abstract

Earlier reports showed that at least 3 herpes simplex virus 1 proteins were adenylylated and guanylylated in addition to the infected cell proteins (ICPs) 0, 4, 22, and 27. ICP22 and 27 share the amino acid sequence R/PRAP/SR which is also predicted to be in ICPs 4 and 0 and in the products of the HSV-1 genes UL21, UL31, UL47, and UL49. ICPs 0, 4, 22, and 27 are regulatory proteins, UL21 is dispensable for growth in cultured cells, UL31 co-fractionates with the nuclear matrix, and UL47 may interact stoichiometrically with the alpha trans-inducing factor (VP16, the product of the UL48 gene). To avoid bias, the genes encoding the additional nucleotidylylated proteins were mapped, initially by analyses of intertypic recombinant viruses, and subsequently by analyses of the products encoded by the mapped genome domains. We conclude that (i) the products of UL21, UL31, UL47, and UL49 are nucleotidylylated by [alpha-32P]GTP or [alpha-32P]ATP in isolated nuclei, (ii) this modification does not involve the viral protein kinases encoded by the UL13 and US3 gene products and, (iii) UL49 is a virion protein which is labeled in cells with [32P]orthophosphate and also is ADP-ribosylated.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources