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. 1994 Mar;136(3):1121-41.
doi: 10.1093/genetics/136.3.1121.

Variegated phenotype and developmental methylation changes of a maize allele originating from epimutation

O P Das  1 , J Messing
Affiliations

Variegated phenotype and developmental methylation changes of a maize allele originating from epimutation

O P Das et al. Genetics. 1994 Mar.

Abstract

Two instances of genetic transmission of spontaneous epimutation of the maize P-rr gene were identified. Transmission gave rise to two similar, moderately stable alleles, designated P-pr-1 and P-pr-2, that exhibited Mendelian behavior. Both isolates of P-pr conditioned a variable and variegated phenotype, unlike the uniform pigmentation conditioned by P-rr. Extensive genomic analysis failed to reveal insertions, deletions or restriction site polymorphisms between the new allele and its progenitor. However, methylation of the P gene was increased in P-pr relative to P-rr, and was greatly reduced (though not lost) in a revertant to uniform pigmentation. Variability in pigmentation conditioned by P-pr correlated with variability in transcript levels of the P gene, and both correlated inversely with variability in its methylation. Part of the variability in methylation could be accounted for by a developmental decrease in methylation in all tissues of plants carrying P-pr. We hypothesize that the variegated phenotype results from a general epigenetic pathway which causes a progressive decrease in methylation and increase in expression potential of the P gene as a function of cell divisions in each meristem of the plant. This renders all tissues chimeric for a functional gene; chimerism is visualized as variegation only in pericarp due to the tissue specificity of P gene expression. Therefore, this allele that originates from epimutation may exemplify an epigenetic mechanism for variegation in maize.

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References

    1. Proc Natl Acad Sci U S A. 1990 Oct;87(20):7809-13 - PubMed
    1. Annu Rev Genet. 1973;7:129-52 - PubMed
    1. Genes Dev. 1990 Mar;4(3):331-43 - PubMed
    1. Bioessays. 1989 May;10(5):139-44 - PubMed
    1. Mol Gen Genet. 1991 Oct;229(3):365-72 - PubMed

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