Apoptotic cell death of a hybrid motoneuron cell line induced by immunoglobulins from patients with amyotrophic lateral sclerosis
- PMID: 7964760
- DOI: 10.1046/j.1471-4159.1994.63062365.x
Apoptotic cell death of a hybrid motoneuron cell line induced by immunoglobulins from patients with amyotrophic lateral sclerosis
Abstract
Apoptotic cell death has recently been implicated in diseases involving nonproliferating, terminally differentiated cells such as neurons. Previous experiments have documented that immunoglobulins from patients with amyotrophic lateral sclerosis (ALS) can kill motoneuron-neuroblastoma hybrid cells [ventral spinal cord 4.1 (VSC 4.1)] by a calcium-dependent process. Here, we studied the mechanism of ALS IgG-induced cell death. In the presence of ALS IgG the VSC 4.1 cells undergo cell shrinkage and membrane blebbing, which are morphological features of apoptotic cell death. The damaged cells can be identified by in situ end labeling of nicked DNA and biochemically show laddering on agarose gel electrophoresis. This ALS IgG-triggered process is prevented by cycloheximide, aurintricarboxylic acid, and zinc sulfate. These data demonstrate that immunoglobulins from patients with ALS are able to induce apoptosis in motoneuron hybrid cells and provide a potential mechanism for motoneuron degeneration in human ALS.
Similar articles
-
The role of calcium-binding proteins in selective motoneuron vulnerability in amyotrophic lateral sclerosis.Ann Neurol. 1994 Dec;36(6):846-58. doi: 10.1002/ana.410360608. Ann Neurol. 1994. PMID: 7998770
-
Cytotoxicity of immunoglobulins from amyotrophic lateral sclerosis patients on a hybrid motoneuron cell line.Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3393-7. doi: 10.1073/pnas.91.8.3393. Proc Natl Acad Sci U S A. 1994. PMID: 8159758 Free PMC article.
-
Expression of calbindin-D28K in motoneuron hybrid cells after retroviral infection with calbindin-D28K cDNA prevents amyotrophic lateral sclerosis IgG-mediated cytotoxicity.Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6796-801. doi: 10.1073/pnas.93.13.6796. Proc Natl Acad Sci U S A. 1996. PMID: 8692898 Free PMC article.
-
Autoimmunity and ALS.Neurology. 1996 Oct;47(4 Suppl 2):S40-5; discussion S45-6. doi: 10.1212/wnl.47.4_suppl_2.40s. Neurology. 1996. PMID: 8858050 Review.
-
Evidence for autoimmunity in amyotrophic lateral sclerosis.J Neurol Sci. 1993 Sep;118(2):169-74. doi: 10.1016/0022-510x(93)90106-9. J Neurol Sci. 1993. PMID: 8229065 Review.
Cited by
-
Combined Metabolomics and Transcriptomics Approaches to Assess the IL-6 Blockade as a Therapeutic of ALS: Deleterious Alteration of Lipid Metabolism.Neurotherapeutics. 2016 Oct;13(4):905-917. doi: 10.1007/s13311-016-0461-3. Neurotherapeutics. 2016. PMID: 27444617 Free PMC article.
-
Calpain inhibition protected spinal cord motoneurons against 1-methyl-4-phenylpyridinium ion and rotenone.Neuroscience. 2011 Sep 29;192:263-74. doi: 10.1016/j.neuroscience.2011.06.007. Epub 2011 Jun 22. Neuroscience. 2011. PMID: 21723922 Free PMC article.
-
Novel molecular biomarkers at the blood-brain barrier in ALS.Biomed Res Int. 2014;2014:907545. doi: 10.1155/2014/907545. Epub 2014 May 11. Biomed Res Int. 2014. PMID: 24949481 Free PMC article. Review.
-
Pathways and genes differentially expressed in the motor cortex of patients with sporadic amyotrophic lateral sclerosis.BMC Genomics. 2007 Jan 23;8:26. doi: 10.1186/1471-2164-8-26. BMC Genomics. 2007. PMID: 17244347 Free PMC article.
-
Amyotrophic lateral sclerosis: current and future treatment strategies.Drugs. 1996 Jan;51(1):28-44. doi: 10.2165/00003495-199651010-00004. Drugs. 1996. PMID: 8741231 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous
