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. 1994 Dec;127(5):1173-84.
doi: 10.1083/jcb.127.5.1173.

Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae

S M Wilson  1 , K V Datar, M R Paddy, J R Swedlow, M S Swanson
Affiliations

Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae

S M Wilson et al. J Cell Biol. 1994 Dec.

Abstract

To study the functions of heterogeneous nuclear ribonucleoproteins (hnRNPs), we have characterized nuclear polyadenylated RNA-binding (Nab) proteins from Saccharomyces cerevisiae. Nab1p, Nab2p, and Nab3p were isolated by a method which uses UV light to cross-link proteins directly bound to poly(A)+ RNA in vivo. We have previously characterized Nab2p, and demonstrated that it is structurally related to human hnRNPs. Here we report that Nab1p is identical to the Np13p/Nop3p protein recently implicated in both nucleocytoplasmic protein shuttling and pre-rRNA processing, and characterize a new nuclear polyadenylated RNA-binding protein, Nab3p. The intranuclear distributions of the Nab proteins were analyzed by three-dimensional immunofluorescence optical microscopy. All three Nab proteins are predominantly localized within the nucleoplasm in a pattern similar to the distribution of hnRNPs in human cells. The NAB3 gene is essential for cell viability and encodes an acidic ribonucleoprotein. Loss of Nab3p by growth of a GAL::nab3 mutant strain in glucose results in a decrease in the amount of mature ACT1, CYH2, and TPI1 mRNAs, a concomitant accumulation of unspliced ACT1 pre-mRNA, and an increase in the ratio of unspliced CYH2 pre-mRNA to mRNA. These results suggest that the Nab proteins may be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing.

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References

    1. Exp Cell Res. 1973 Aug;80(2):313-21 - PubMed
    1. Trends Genet. 1991 Mar;7(3):79-85 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 - PubMed
    1. J Mol Appl Genet. 1982;1(5):419-34 - PubMed
    1. Nucleic Acids Res. 1983 May 25;11(10):3123-35 - PubMed

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