Two homeo domain proteins bind with similar specificity to a wide range of DNA sites in Drosophila embryos
- PMID: 7958848
- DOI: 10.1101/gad.8.14.1678
Two homeo domain proteins bind with similar specificity to a wide range of DNA sites in Drosophila embryos
Abstract
We have used in vivo UV cross-linking to directly measure DNA binding by the homeo domain proteins even-skipped (eve) and fushi tarazu (ftz) in Drosophila embryos. Strikingly, these two proteins bind at uniformly high levels throughout the length of their genetically identified target genes and at lower, but significant, levels to genes that they are not expected to regulate. The data also suggest that these two proteins have very similar DNA-binding specificities in vivo. In contrast, a non-homeo domain transcription factor, zeste, is only detected on short DNA elements within a target promoter and not on other genes. These results are consistent with the in vitro properties of these various proteins, their respective concentrations in the nucleus, and with earlier predictions of how transcription factors bind DNA in vivo. We propose that these data favor the model that eve, ftz, and closely related homeo domain proteins act by directly regulating mostly the same target genes.
Similar articles
-
DNA binding specificity of two homeodomain proteins in vitro and in Drosophila embryos.Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2680-5. doi: 10.1073/pnas.93.7.2680. Proc Natl Acad Sci U S A. 1996. PMID: 8610101 Free PMC article.
-
The Drosophila nuclear receptors FTZ-F1 alpha and FTZ-F1 beta compete as monomers for binding to a site in the fushi tarazu gene.Mol Cell Biol. 1994 May;14(5):3166-75. doi: 10.1128/mcb.14.5.3166-3175.1994. Mol Cell Biol. 1994. PMID: 8164672 Free PMC article.
-
Accessibility of transcriptionally inactive genes is specifically reduced at homeoprotein-DNA binding sites in Drosophila.Nucleic Acids Res. 2000 Jul 15;28(14):2839-46. doi: 10.1093/nar/28.14.2839. Nucleic Acids Res. 2000. PMID: 10908343 Free PMC article.
-
Target genes of homeodomain proteins.Bioessays. 1999 Apr;21(4):267-70. doi: 10.1002/(SICI)1521-1878(199904)21:4<267::AID-BIES1>3.0.CO;2-C. Bioessays. 1999. PMID: 10377888 Review.
-
Regulation of segmentation and segmental identity by Drosophila homeoproteins: the role of DNA binding in functional activity and specificity.Development. 1997 Nov;124(22):4425-33. doi: 10.1242/dev.124.22.4425. Development. 1997. PMID: 9409661 Review.
Cited by
-
UBF binding in vivo is not restricted to regulatory sequences within the vertebrate ribosomal DNA repeat.Mol Cell Biol. 2002 Jan;22(2):657-68. doi: 10.1128/MCB.22.2.657-668.2002. Mol Cell Biol. 2002. PMID: 11756560 Free PMC article.
-
Chip interacts with diverse homeodomain proteins and potentiates bicoid activity in vivo.Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2686-91. doi: 10.1073/pnas.050586397. Proc Natl Acad Sci U S A. 2000. PMID: 10688916 Free PMC article.
-
A domain of the even-skipped protein represses transcription by preventing TFIID binding to a promoter: repression by cooperative blocking.Mol Cell Biol. 1995 Sep;15(9):4683-93. doi: 10.1128/MCB.15.9.4683. Mol Cell Biol. 1995. PMID: 7651385 Free PMC article.
-
Large-scale turnover of functional transcription factor binding sites in Drosophila.PLoS Comput Biol. 2006 Oct;2(10):e130. doi: 10.1371/journal.pcbi.0020130. Epub 2006 Aug 21. PLoS Comput Biol. 2006. PMID: 17040121 Free PMC article.
-
c-Myc target gene specificity is determined by a post-DNAbinding mechanism.Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13887-92. doi: 10.1073/pnas.95.23.13887. Proc Natl Acad Sci U S A. 1998. PMID: 9811896 Free PMC article.