Review
doi: 10.1016/0168-9525(90)90012-u.
The two-hybrid system: an assay for protein-protein interactions
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- PMID: 7940758
- DOI: 10.1016/0168-9525(90)90012-u
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Review
The two-hybrid system: an assay for protein-protein interactions
Trends Genet.
1994 Aug.
Abstract
The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
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