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Comparative Study
. 1994 Oct 25;91(22):10596-600.
doi: 10.1073/pnas.91.22.10596.

Purification and properties of double-stranded RNA-specific adenosine deaminase from calf thymus

M A O'Connell  1 , W Keller
Affiliations
Comparative Study

Purification and properties of double-stranded RNA-specific adenosine deaminase from calf thymus

M A O'Connell et al. Proc Natl Acad Sci U S A. .

Abstract

A double-stranded RNA-specific adenosine deaminase, which converts adenosine to inosine, has been purified to homogeneity from calf thymus. The enzyme was purified approximately 340,000-fold by a series of column chromatography steps. The enzyme consists of a single polypeptide with a molecular mass of 116 kDa as determined by electrophoresis on a SDS/polyacrylamide gel. The native protein sediments at 4.2 s in glycerol gradients and has a Stokes radius of 42 A upon gel-filtration chromatography. This leads to an estimate of approximately 74,100 for the native molecular weight, suggesting that the enzyme exists as a monomer in solution. Enzyme activity is optimal at 0.1 M KCl and 37 degrees C. Divalent metal ions or ATP is not required for activity. The Km for double-stranded RNA substrate is approximately 7 x 10(-11) M. The Vmax is approximately 10(-9) mol of inosine produced per min per mg and the Kcat is 0.13 min-1.

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