Developmentally excised DNA sequences in Euplotes crassus capable of forming G quartets
- PMID: 7892211
- PMCID: PMC42406
- DOI: 10.1073/pnas.92.6.1979
Developmentally excised DNA sequences in Euplotes crassus capable of forming G quartets
Abstract
Tens of thousands of DNA segments are eliminated by DNA breakage and rejoining events during the formation of a new macronucleus in the hypotrichous ciliated protozoan Euplotes crassus. This study presents evidence for a class of eliminated sequences referred to as telomeric-repeat-like internal eliminated sequences (TelIESs). TelIESs are shorter (< 50 bp) than most previously characterized IESs and their DNA sequences resemble the telomeric repeat sequences of the organism. The TelIESs are excised during the developmental period of chromosome fragmentation/telomere addition, which is later than previously characterized IESs. Additional studies demonstrate that oligonucleotides representing the TelIESs are, like telomeric repeats, capable of forming G-quartet structures in vitro.
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