doi: 10.1126/science.7809612.
Structure-based design of transcription factors
Affiliations
- PMID: 7809612
- DOI: 10.1126/science.7809612
Item in Clipboard
Structure-based design of transcription factors
Science.
.
Abstract
Computer modeling suggested that transcription factors with novel sequence specificities could be designed by combining known DNA binding domains. This structure-based strategy was tested by construction of a fusion protein, ZFHD1, that contained zinc fingers 1 and 2 from Zif268, a short polypeptide linker, and the homeodomain from Oct-1. The fusion protein bound optimally to a sequence containing adjacent homeodomain (TAATTA) and zinc finger (NGGGNG) subsites. When fused to an activation domain, ZFHD1 regulated promoter activity in vivo in a sequence-specific manner. Analysis of known protein-DNA complexes suggests that many other DNA binding proteins could be designed in a similar fashion.
Similar articles
-
Analysis of homeodomain function by structure-based design of a transcription factor.Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9752-6. doi: 10.1073/pnas.92.21.9752. Proc Natl Acad Sci U S A. 1995. PMID: 7568211 Free PMC article.
-
Combination of a zinc finger and homeodomain required for protein-interaction.Mol Biol Rep. 2003 Dec;30(4):199-206. doi: 10.1023/a:1026330907065. Mol Biol Rep. 2003. PMID: 14672405 Free PMC article.
-
Co-expression of the stress-inducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis.Plant J. 2007 Jan;49(1):46-63. doi: 10.1111/j.1365-313X.2006.02932.x. Plant J. 2007. PMID: 17233795
-
The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain.Genes Dev. 1995 Jul 15;9(14):1679-93. doi: 10.1101/gad.9.14.1679. Genes Dev. 1995. PMID: 7622033 Review. No abstract available.
-
Custom DNA-binding proteins and artificial transcription factors.Curr Top Med Chem. 2003;3(6):645-57. doi: 10.2174/1568026033452384. Curr Top Med Chem. 2003. PMID: 12570857 Review.
Cited by
-
Characterisation of site-biased DNA methyltransferases: specificity, affinity and subsite relationships.Nucleic Acids Res. 2002 Sep 1;30(17):3818-30. doi: 10.1093/nar/gkf501. Nucleic Acids Res. 2002. PMID: 12202767 Free PMC article.
-
A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system.BMC Biotechnol. 2004 Apr 29;4:9. doi: 10.1186/1472-6750-4-9. BMC Biotechnol. 2004. PMID: 15117411 Free PMC article.
-
Computational prediction of transcription-factor binding site locations.Genome Biol. 2003;5(1):201. doi: 10.1186/gb-2003-5-1-201. Epub 2003 Dec 23. Genome Biol. 2003. PMID: 14709165 Free PMC article. Review.
-
Protein binding microarrays for the characterization of DNA-protein interactions.Adv Biochem Eng Biotechnol. 2007;104:65-85. doi: 10.1007/10_025. Adv Biochem Eng Biotechnol. 2007. PMID: 17290819 Free PMC article. Review.
-
Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication.Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5640-4. doi: 10.1073/pnas.92.12.5640. Proc Natl Acad Sci U S A. 1995. PMID: 7777563 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
