Flavohaemoglobin HmpX: a new pathogenicity determinant in Erwinia chrysanthemi strain 3937
- PMID: 7773389
- DOI: 10.1099/13500872-141-4-863
Flavohaemoglobin HmpX: a new pathogenicity determinant in Erwinia chrysanthemi strain 3937
Abstract
Unlike wild-type Erwinia chrysanthemi strain 3937, which fully macerates inoculated Saintpaulia plants, HmpX- mutants produce necrotic lesions or no symptoms. The hmpX gene was sequenced and the corresponding protein sequence analysed. We show that HmpX belongs to a family of flavohaemoproteins (HMP), previously identified in two yeasts and in Escherichia coli. Comparisons of protein sequences at the secondary structure level by hydrophobic cluster analysis have shown that HmpX possesses two functional regions, a haemoglobin domain in its N-terminal part and a flavin reductase domain in its C-terminal part. In an HmpX- strain, the synthesis of pectate lyases, which are pathogenicity determinants in E. chrysanthemi, was reduced in conditions of low oxygen tension. Using gus fusion in hmpX, it was shown that hmpX transcription was induced in coculture with tobacco cells. A putative function for HmpX is discussed.
Similar articles
-
The Erwinia chrysanthemi EC16 hrp/hrc gene cluster encodes an active Hrp type III secretion system that is flanked by virulence genes functionally unrelated to the Hrp system.Mol Plant Microbe Interact. 2004 Jun;17(6):644-53. doi: 10.1094/MPMI.2004.17.6.644. Mol Plant Microbe Interact. 2004. PMID: 15195947
-
Role of the nucleoid-associated protein H-NS in the synthesis of virulence factors in the phytopathogenic bacterium Erwinia chrysanthemi.Mol Plant Microbe Interact. 2001 Jan;14(1):10-20. doi: 10.1094/MPMI.2001.14.1.10. Mol Plant Microbe Interact. 2001. PMID: 11194867
-
Erwinia chrysanthemi hrp genes and their involvement in soft rot pathogenesis and elicitation of the hypersensitive response.Mol Plant Microbe Interact. 1994 Sep-Oct;7(5):573-81. doi: 10.1094/mpmi-7-0573. Mol Plant Microbe Interact. 1994. PMID: 7949326
-
hrp genes of Erwinia chrysanthemi 3937 are important virulence factors.Mol Plant Microbe Interact. 2002 May;15(5):472-80. doi: 10.1094/MPMI.2002.15.5.472. Mol Plant Microbe Interact. 2002. PMID: 12036278
-
Flavohaemoglobin HmpX from Erwinia chrysanthemi confers nitrosative stress tolerance and affects the plant hypersensitive reaction by intercepting nitric oxide produced by the host.Plant J. 2005 Jul;43(2):226-37. doi: 10.1111/j.1365-313X.2005.02443.x. Plant J. 2005. PMID: 15998309
Cited by
-
Novel flavohemoglobins of mycobacteria.IUBMB Life. 2011 May;63(5):337-45. doi: 10.1002/iub.460. Epub 2011 Apr 13. IUBMB Life. 2011. PMID: 21491561 Free PMC article.
-
Paraquat regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12 is SoxRS independent but modulated by sigma S.J Bacteriol. 1997 May;179(10):3164-70. doi: 10.1128/jb.179.10.3164-3170.1997. J Bacteriol. 1997. PMID: 9150210 Free PMC article.
-
PehN, a polygalacturonase homologue with a low hydrolase activity, is coregulated with the other Erwinia chrysanthemi polygalacturonases.J Bacteriol. 2002 May;184(10):2664-73. doi: 10.1128/JB.184.10.2664-2673.2002. J Bacteriol. 2002. PMID: 11976295 Free PMC article.
-
Nitric oxide in plant immunity.Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10345-7. doi: 10.1073/pnas.95.18.10345. Proc Natl Acad Sci U S A. 1998. PMID: 9724703 Free PMC article. No abstract available.
-
Protection from nitrosative stress by yeast flavohemoglobin.Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4672-6. doi: 10.1073/pnas.090083597. Proc Natl Acad Sci U S A. 2000. PMID: 10758168 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
