Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 May 9;92(10):4289-93.
doi: 10.1073/pnas.92.10.4289.

Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle

K E Sawin  1 , T J Mitchison
Affiliations

Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle

K E Sawin et al. Proc Natl Acad Sci U S A. .

Abstract

Eg5, a member of the bimC subfamily of kinesin-like microtubule motor proteins, localizes to spindle microtubules in mitosis but not to interphase microtubules. We investigated the molecular basis for spindle localization by transient transfection of Xenopus A6 cells with myc-tagged derivatives of Eg5. Expressed at constitutively high levels from a cytomegalovirus promoter, mycEg5 protein is cytoplasmic throughout interphase, begins to bind microtubules in early prophase, and remains localized to spindle and/or midbody microtubules through mitosis to the end of telophase. Both N- and C-terminal regions of Eg5 are required for this cell-cycle-regulated targeting. Eg5 also contains within its C-terminal domain a sequence conserved among bimC subfamily proteins that includes a potential p34cdc2 phosphorylation site. We show that mutation of a single threonine (T937) within this site to nonphosphorylatable alanine abolishes localization of the mutant protein to the spindle, whereas mutation of T937 to serine preserves spindle localization. We hypothesize that phosphorylation of Eg5 may regulate its localization to the spindle in the cell cycle.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Dev Biol. 1994 Jul;164(1):147-59 - PubMed
    1. J Cell Biol. 1994 Feb;124(4):507-19 - PubMed
    1. Nature. 1992 Oct 8;359(6395):540-3 - PubMed
    1. J Biol Chem. 1991 Aug 15;266(23):15180-4 - PubMed
    1. Curr Opin Cell Biol. 1994 Feb;6(1):69-73 - PubMed

Publication types

MeSH terms

LinkOut - more resources