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. 1995 Mar 15;14(6):1057-66.
doi: 10.1002/j.1460-2075.1995.tb07088.x.

A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae

H Interthal  1 , C Bellocq, J Bähler, V I Bashkirov, S Edelstein, W D Heyer
Affiliations

A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae

H Interthal et al. EMBO J. .

Abstract

Saccharomyces cerevisiae cells lacking the SEP1 (also known as XRN1, KEM1, DST2, RAR5) gene function exhibit a number of phenotypes in cellular processes related to microtubule function. Mutant cells show increased sensitivity to the microtubule-destabilizing drug benomyl, increased chromosome loss, a karyogamy defect, impaired spindle pole body separation, and defective nuclear migration towards the bud neck. Analysis of the arrest morphology and of the survival during arrest strongly suggests a structural defect accounting for the benomyl hypersensitivity, rather than a regulatory defect in a checkpoint. Biochemical analysis of the purified Sep1 protein demonstrates its ability to promote the polymerization of procine brain and authentic S.cerevisiae tubulin into flexible microtubules in vitro. Furthermore, Sep1 co-sediments with these microtubules in sucrose cushion centrifugation. Genetic analysis of double mutant strains containing a mutation in SEP1 and in one of the genes coding for alpha- or beta-tubulin further suggests interaction between Sep1 and microtubules. Taken together these three lines of evidence constitute compelling evidence for a role of Sep1 as an accessory protein in microtubule function in the yeast S.cerevisiae.

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References

    1. Eur J Cell Biol. 1990 Apr;51(2):295-302 - PubMed
    1. J Biol Chem. 1990 Jul 5;265(19):10968-73 - PubMed
    1. J Cell Biol. 1990 Dec;111(6 Pt 1):2573-86 - PubMed
    1. Genetics. 1990 Dec;126(4):799-812 - PubMed
    1. Methods Enzymol. 1991;194:428-53 - PubMed

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