Distinct NF-kappa B/Rel transcription factors are responsible for tissue-specific and inducible gene activation
- PMID: 7692309
- DOI: 10.1038/365767a0
Distinct NF-kappa B/Rel transcription factors are responsible for tissue-specific and inducible gene activation
Abstract
The NF-kappa B/Rel family is a growing class of transcriptional regulators whose members share the conserved Rel-homology domain, involved in specific DNA binding and dimerization. They interact with the regulatory elements of many different genes and are involved in the regulation of lymphoid-specific and inducible transcription. We tested whether these factors could alone activate a gene in transgenic mice. We report here that a minimal promoter containing three copies of a binding site for these proteins allows tissue-specific and inducible transgene activation. In lymphoid tissues constitutive transgene expression correlates with the presence of a constitutively active p50/RelB heterodimer. Other organs that only contain the p50 homodimer do not express the transgene. In contrast to this constitutive activity mediated by p50/RelB, the p50/p65 heterodimer (which is NF-kappa B) could confer inducible transgene activation in embryo fibroblasts. Thus two different members of the NF-kappa B/Rel family of transcriptional activators are involved in tissue-specific and inducible gene activation in transgenic mice.
Similar articles
-
Activation of multiple NF-kappa B/Rel DNA-binding complexes by tumor necrosis factor.Oncogene. 1994 May;9(5):1487-92. Oncogene. 1994. PMID: 8152812
-
Promoter of the human NF-kappa B p50/p105 gene. Regulation by NF-kappa B subunits and by c-REL.J Immunol. 1993 Apr 1;150(7):2794-804. J Immunol. 1993. PMID: 8454856
-
Regulation of intercellular adhesion molecule-1 gene by tumor necrosis factor-alpha is mediated by the nuclear factor-kappaB heterodimers p65/p65 and p65/c-Rel in the absence of p50.Cell Growth Differ. 1997 Mar;8(3):335-42. Cell Growth Differ. 1997. PMID: 9056676
-
RelB, a member of the Rel/NF-kappa B family of transcription factors.Braz J Med Biol Res. 1996 Jul;29(7):895-903. Braz J Med Biol Res. 1996. PMID: 9070378 Review.
-
The role of NF-κB activation during protection against Leishmania infection.Int J Med Microbiol. 2012 Oct;302(4-5):230-5. doi: 10.1016/j.ijmm.2012.07.006. Epub 2012 Aug 14. Int J Med Microbiol. 2012. PMID: 22901377 Review.
Cited by
-
Constitutive nuclear NF kappa B/rel DNA-binding activity of rat thymocytes is increased by stimuli that promote apoptosis, but not inhibited by pyrrolidine dithiocarbamate.Biochem J. 1995 Dec 15;312 ( Pt 3)(Pt 3):833-8. doi: 10.1042/bj3120833. Biochem J. 1995. PMID: 8554528 Free PMC article.
-
TNF-α Modulation of Intestinal Tight Junction Permeability Is Mediated by NIK/IKK-α Axis Activation of the Canonical NF-κB Pathway.Am J Pathol. 2016 May;186(5):1151-65. doi: 10.1016/j.ajpath.2015.12.016. Epub 2016 Mar 4. Am J Pathol. 2016. PMID: 26948423 Free PMC article.
-
Modulation of the NFκb Signalling Pathway by Human Cytomegalovirus.Virology (Hyderabad). 2017 Aug;1(1):104. Epub 2017 Jul 31. Virology (Hyderabad). 2017. PMID: 29082387 Free PMC article.
-
NF-kappaB controls the global pro-inflammatory response in endothelial cells: evidence for the regulation of a pro-atherogenic program.Nucleic Acids Res. 2005 Sep 21;33(16):5308-19. doi: 10.1093/nar/gki836. Print 2005. Nucleic Acids Res. 2005. PMID: 16177180 Free PMC article.
-
Btk expression is controlled by Oct and BOB.1/OBF.1.Nucleic Acids Res. 2006 Mar 31;34(6):1807-15. doi: 10.1093/nar/gkl131. Print 2006. Nucleic Acids Res. 2006. PMID: 16582106 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
