Comparative Study
doi: 10.1006/abio.1993.1253.
Covalent attachment of peptides to membranes for dot-blot analysis of glycosylation sites and epitopes
Affiliations
- PMID: 7686351
- DOI: 10.1006/abio.1993.1253
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Comparative Study
Covalent attachment of peptides to membranes for dot-blot analysis of glycosylation sites and epitopes
Anal Biochem.
1993 Jun.
Abstract
Noncovalent binding of proteins to membranes is often employed for dot-blot analysis with various visualization techniques. These techniques are usually not applicable to peptide dot-blot analysis due to peptide wash-off during the staining procedure. As exemplified with a synthetic peptide and peptides produced by proteolysis of a protein, it is possible to achieve efficient covalent attachment to Immobilon-AV membranes. The utility of this membrane has been demonstrated with immunostaining and carbohydrate staining procedures.
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