Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Jun;67(6):3489-96.
doi: 10.1128/JVI.67.6.3489-3496.1993.

Mutational analysis of the envelope gene of Moloney murine leukemia virus

K D Gray  1 , M J Roth
Affiliations

Mutational analysis of the envelope gene of Moloney murine leukemia virus

K D Gray et al. J Virol. 1993 Jun.

Abstract

The env gene products of Moloney murine leukemia virus are required for binding and entry of the virus into the target cell. Thirty-three linker insertion mutations were constructed throughout the env gene of Moloney murine leukemia virus. Twenty of the mutations were located in the surface protein (SU), and the remaining thirteen were located in the transmembrane protein (TM). The viability of the viruses containing these env gene mutations was determined by performing transient transfections and screening for the release of reverse transcriptase. Eleven viable mutants were isolated, nine in SU and two in TM. Three of the viable mutants were temperature sensitive. Four of the viable mutants were clustered in the carboxy terminus of SU. The env gene products of transfected cell lines which produced viable virus were analyzed. Our results indicated two regions of SU important for the stability of the SU/TM heteropolymer and one region important for the interaction of the env gene products with the viral core.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
    1. J Virol. 1992 Nov;66(11):6616-25 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Virology. 1970 Dec;42(4):1136-9 - PubMed
    1. J Virol. 1981 Apr;38(1):239-48 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources