Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Feb;120(4):1003-10.
doi: 10.1083/jcb.120.4.1003.

Spreading of human endothelial cells on fibronectin or vitronectin triggers elevation of intracellular free calcium

M A Schwartz  1
Affiliations

Spreading of human endothelial cells on fibronectin or vitronectin triggers elevation of intracellular free calcium

M A Schwartz. J Cell Biol. 1993 Feb.

Abstract

Intracellular calcium ([Ca2+]i) was measured in FURA 2-loaded endothelial cells plated on fibronectin or vitronectin. Average values for [Ca2+]i increased to approximately twofold above basal levels by approximately 1 h after plating, and then declined. The increase in [Ca2+]i required extracellular calcium. Substituting potassium for sodium in the medium reduced the elevation of [Ca2+]i, a result that rules out the involvement of Na-Ca exchangers or voltage-dependent calcium channels, but that is consistent with the involvement of voltage-independent calcium channels. Plating cells on an anti-integrin beta 1 subunit antibody gave a similar [Ca2+]i response, but clustering beta 1 integrins with the same antibody, or occupying integrins with RGD (arg-gly-asp) peptides had no effect. Time course measurements on single cells revealed that in each cell [Ca2+]i rose abruptly at some point during spreading, from the basal level to a higher steady-state level that was maintained for some time. The elevated [Ca2+]i was unrelated to previously observed changes in intracellular pH, because chelating the Ca2+ in the medium failed to inhibit the elevation of pHi that occurred during cell spreading. In conclusion, these results show that integrin-mediated cell spreading can regulate [Ca2+]i, and the pathways involved are distinct from those that regulate intracellular pH.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Thromb Res. 1982 Jul 1;27(1):1-14 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6138-41 - PubMed
    1. J Cell Biol. 1987 Dec;105(6 Pt 1):2543-9 - PubMed
    1. J Cell Biol. 1988 Jul;107(1):321-31 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4525-9 - PubMed

Publication types