doi: 10.1016/0014-5793(93)81507-v.
Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2
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- PMID: 7679080
- DOI: 10.1016/0014-5793(93)81507-v
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Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2
FEBS Lett.
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Free article
Abstract
A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.
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