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. 1995 Sep;69(9):5816-20.
doi: 10.1128/JVI.69.9.5816-5820.1995.

Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form

S L Allison  1 , K Stadler, C W Mandl, C Kunz, F X Heinz
Affiliations

Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form

S L Allison et al. J Virol. 1995 Sep.

Abstract

A quantitative study was performed to investigate the requirements for secretion of recombinant soluble and particulate forms of the envelope glycoprotein E of tick-borne encephalitis (TBE) virus. Full-length E and a carboxy terminally truncated anchor-free form were expressed in COS cells in the presence and absence of prM, the precursor of the viral membrane protein M. Formation of a heteromeric complex with prM was found to be necessary for efficient secretion of both forms of E, whereas only low levels of anchor-free E were secreted in the absence of prM. The prM-mediated transport function could also be provided by coexpression of prM and E from separate constructs, but a prM-to-E ratio of greater than 1:1 did not further enhance secretion. Full-length E formed stable intracellular heterodimers with prM and was secreted as a subviral particle, whereas anchor-free E was not associated with particles and formed a less stable complex with prM, suggesting that prM interacts with both the ectodomain and anchor region of E.

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